引用本文:苏英锋 1,孙秀珍 1,刘迎曦 2,闫志勇 1,沈双 2,辛晓燕 1,于 申 2.豚鼠耳蜗生物数值模型的实验研究[J].大连医科大学学报,2015,37(4):324-327.
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豚鼠耳蜗生物数值模型的实验研究
苏英锋 1,孙秀珍 1,刘迎曦 2,闫志勇 1,沈双 2,辛晓燕 1,于 申 21,2
1.大连医科大学附属第二医院 耳鼻喉科,辽宁 大连 116027;2.大连理工大学 工业装备结构分析国家重点实验室,辽宁 大连 116024
摘要:
[摘要] 目的 建立豚鼠耳蜗宏观生物数值模型。 方法 取成年豚鼠1只,进行电耳镜、声导抗、听性脑干反应、耳声发射及内耳薄层CT扫描,除外耳部疾患及内耳畸形,全麻断头后取耳蜗,经石蜡包埋、连续切片(6 μm)、HE染色、摄像等步骤获得一整套豚鼠内耳组织切片的图像资料;利用Photoshop软件进行去色、调整灰度及对比度等处理,利用mimics软件识别边界并标定,获得边界点的三维坐标,将坐标导入Hypermesh软件建立网格化模型,利用ANASYS软件建立耳蜗膜迷路的三维生物数值模型,基于该模型进行基底膜的模态分析。 结果 所建豚鼠耳蜗生物数值模型能够清晰显示耳蜗的空间结构特性;生物数值模拟显示了耳蜗基底膜前10阶感音频率特异性,与“行波学说”结果一致。 结论 运用组织连续切片技术并结合有限元分析软件建立耳蜗宏观生物数值模型的方法可行,所建模型可用于耳蜗感音功能研究。
关键词:  生物数值模型  耳蜗  组织切片  数值模拟
DOI:10.11724/jdmu.2015.04.03
分类号:
基金项目:基金项目:国家自然科学基金项目(11472047);辽宁省教育厅基金项目(L2012323)
Establishment of the biomechanical model of cochlea of guinea pig
SU Ying-feng 1, SUN Xiu-zhen 1, LIU Ying-xi 2, YAN Zhi-yong 1, SHEN Shuang 2, XIN Xiao-yan 1, YU Shen 21,2
1.Department of Otorhinalaryngology, the Second Affiliated Hospital of Dalian Medical University, Dalian 116027, China;2.State Key Laboratory of Structural Analysis for Industrial Equipment, Department of Engineering Mechanicals, Dalian University of Technology, Dalian 116024, China
Abstract:
[Abstract] Objective To establish a macro-biomechanical model of the guinea pig's cochlea. Methods Both cochlea, abstracted from bilateral temporal bones of a healthy adult guinea pig, which was tested firstly including a series of examination including electric otoscopy, acoustic immittance,brainstem auditory evoked potential, otoacoustic emissions, CT scan and so on.And then, a whole set of inner ear tissue slices images was obtained after a series of operations including embedding, slicing (thickness 6 μm), immobilization, coloretur, photographing and so on. The images were processed by Photoshop software, mimics software and Hypermesh software sequencely and a biomechanical model of the cochlea was obtained with ANSYS13.0 eventually. Based on the model, the modal analysis was done to test the accuracy of the model. Results  Spatial structural feature of the reconstructed biomechanical model of the guinea pig's cochlea was displayed typically and the result of the model analysis proceeded on the macro-biomechanical model was the same as traveling wave theory. Conclusion It is feasible technically to reconstruct biomechanical model of the cochlea with the tissue slices combining with the finite element soft wares. And the macro-biomechanical model could be applied to study the sensing process of cochlea.
Key words:  [Key words] biomechanical model  cochlea  tissue slice  numerical simulation