R(+)普拉克索对急性脑缺血大鼠脑损伤的保护作用

Protective effects of R(+) pramipexole on acute cerebral ischemia injury in rats

关键词:  R(+)普拉克索  急性脑缺血  梗死体积  氧自由基

Key words:  [Key words]　R(+) pramipexole  acute cerebral ischemia  infarct volume  reactive oxygen species

摘要:

[摘要]　目的　研究R(+)普拉克索(R+PPX)对急性脑缺血大鼠体内氧自由基生成的影响，及其对急性脑缺血大鼠脑组织的保护作用。　方法　采用线栓法建立大鼠脑缺血2 h再灌注模型，抽签方法均分60只大鼠为3组：假手术（sham）组，中动脉栓塞（MCAO）组， R+PPX注射中动脉栓塞（R+PPX+MCAO）组。于再灌注8 h和24 h时，采用TTC磷酸盐染色、LOZEX-F图像扫描确定脑梗死体积，用新型荧光探针H2DCF-DA监测大鼠脑内氧自由基（ROS）阳性细胞数目。　结果 　（1）sham组大鼠未出现脑梗死，再灌注8 h和24 h时，MCAO、R+PPX+MCAO两组均出现脑梗死（P<0.05）。再灌注8 h时，R+PPX+MCAO组样本的脑梗死体积明显小于MCAO组（P<0.05），但再灌注24 h时，MCAO、R+PPX+MCAO两组的脑梗死体积无明显差异。（3）再灌注8 h时，R+PPX+MCAO组的ROS阳性细胞数明显少于MCAO组（P<0.05），但再灌注24 h时，这两组的ROS阳性细胞数差异无显著性意义。　结论　R+PPX干预可以明显减少再灌注早期脑缺血大鼠组织ROS细胞生成及减少脑梗死体积。

Abstract:

[Abstract]　Objective　To study the effects of R (+) pramipexole (R+PPX) on the generation of reactive oxygen species(ROS) and to investigate the protective effects of R+PPX on brain tissue after cerebral ischemia in rats and provide experimental basis for effective treatment for ischemic stroke.　Methods　The rat model of cerebral ischemia for 2 h and reperfusion was established by thread occlusion. Ninty rats were divided into three groups randomly: sham group，middle cerebral artery occlusion (MCAO) group and the R+PPX+MCAO group. After reperfusion for 8 h and 24 h, the volume of cerebral infarct was determined by the TTC and LOZEX-F image scanning, and the ROS positive cell number was monitored with the new fluorescent probe H2DCF-DA.　Results　No cerebral infarction was observed in the sham group but the cerebral infarct volume increased significantly after 8 h and 24 h reperfusion in both MCAO group and R+PPX+MCAO group(P<0.05). After reperfusion for 8 h, the cerebral infarct volume in R+PPX+MCAO group was higher and the number of H2DCFA-DA positive cells was lower than that in MCAO group respectively (P<0.05), while both of them were not statistical different between the two groups after reperfusion for 24 h (P>0.05).　Conclusion　R+PPX intervention might reduce the cerebral infarct volume and protect the brain by decreasing the generation of ROS cells in early stage of cerebral ischemia in rats.