电针次髎穴对慢性膀胱过度活动症模型大鼠尿动力学及脊髓背角SP和CGRP影响

Urodynamic effects of electroacupuncture at Ciliao on overactive bladder in rats and CGRP, SP expressions of dorsal horn of spinal cord

关键词:  电针  膀胱过度活动症  P物质  降钙素基因相关肽  尿动力学

Key words:  electroacupuncture  overactive bladder  substance P  calcitonin gene-elated peptide  urodynamic

摘要:

[目的] 探讨电针次髎穴对环磷酰胺(cyclophsphamide，CYP)致慢性膀胱过度活动症（overactive bladder, OAB）模型大鼠尿动力学及脊髓背角p物质（substance P, SP)和降钙素基因相关肽(calcitonin-gene related peptide, CGRP)的影响。[方法] 雌性大鼠25只随机分为对照组5只，OAB模型组10只，OAB+电针(电针组)10只。其中模型组和电针组动物腹腔内注射环磷酰胺诱导OAB，应用膀胱测压技术动态观察电针次髎穴后排尿间期、基础膀胱压、膀胱最大充盈压及最大排尿压的变化；应用免疫组织化学方法定量观察电针后脊髓背角SP 和CGRP表达变化。[结果] 电针组和模型组的排尿间期与对照组相比明显缩短(P<0.05),基础膀胱压明显升高(P<0.01)。电针后电针组的基础膀胱压较模型组明显降低(P<0.01),排尿间期较模型组明显延长(P<0.01)。与对照组相比，模型组脊髓后角SP及CGRP表达明显升高(P<0.05)；与模型组相比，电针组的脊髓后角SP及CGRP表达降低(P<0.05)。 [结论] 电针次髎穴能抑制环磷酰胺诱导的大鼠膀胱过度活动，降低脊髓后角SP和CGRP的表达，电针次髎穴治疗OAB可能与抑制膀胱传入神经的兴奋性及传入递质的释放与合成有关。

Abstract:

[Objectives] To investigate the urodynamic effects of electroacupuncture at CiLiao (BL 32) on overactive bladder(OAB) in rats induced by cyclophsphamide（CYP）and expressions of calcitonin-gene related peptide（CGRP）and SP in dorsal horn of spinal cord. [Methods] Twenty five Sprague-Dawley rats were randomly divided into: control group (n=5), chronic OAB group (n=10), chronic OAB+electroacupuncture group (n=10). OAB model was established by intraperitoneal injection (i. p. i) of cyclophsphamide，continuous cystometry was performed to detectinter-contraction interval，basal, threshold, micturition voiding pressure before and after electroacupuncture. The expressions of CGRP and SP in the dorsal horn of spinal cord were detected by immunohistochemical method. [Results] Compared with control group, inter-contraction interval was decreased (P<0.05) and basal pressure was increased (P<0.01) in both OAB and ele ctroacupuncture group before acupuncture. While basal pressure was decreased and inter-contraction interval was increased significantly in acupuncture group compared with those in control group (P<0.01). Comparison with control group, the mean optical density (OD) value of SP and CGRP positive products in the dorsal horn of sacral cord in OAB group was increased significantly (P<0.05), while compared with OAB group, the mean OD value of SP and CGRP in acupuncture group was decreased(P<0.05). [Conclusions] Electroacupuncture at CiLiao could inhibit the overactive bladder induced by cyclophsphamide in rats by decreasing the SP and CGRP expressions in the dorsal horn of spinal cord.