| 摘要: |
| [目的] 了解Rad18敲除雄小鼠生殖细胞的变化。[方法] 用免疫组织化学方法检测2、6、12月龄Rad18-/-雄小鼠生精小管生殖细胞的特异性标记物TRA98的表达及分布，并与野生（WT）雄小鼠比较；用原位杂交技术检测Rad18在1周龄WT雄小鼠生精小管未分化精原细胞中的表达。[结果] 缺失TRA98标记的早期精原细胞的生精小管比例在12月龄Rad18-/-和WT雄小鼠中分别为39.50%和2.76%，差异有显著性意义（P<0.05）；但Rad18-/-退化生精小管中保留正常的Sertoli细胞，其附睾中未见TRA98阳性的生殖细胞，且精子形态正常；1周龄WT小鼠睾丸未分化精原细胞中有Rad18蛋白表达。[结论] Rad18可能对精原干细胞有维持作用。 |
| 关键词: Rad18-/-小鼠 TRA98 精原干细胞 |
| DOI：10.11724/jdmu.2012.03.05 |
| 分类号: |
| 基金项目:大连医科大学附属第二医院青年基金项目（2009） |
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| Effect of Rad18 deletion on germ cells of male mice |
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SUN Jing-hua 1， LIU Li-feng 21,2
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1.Department of Oncology, the Second Affiliated Hospital of Dalian Medical University,Dalian 116027,China;2.Department of Gynecology and Obstetrics, the First Affiliated Hospital of Dalian Medical University, Dalian 116011,China
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| Abstract: |
| [Objective] To investigate the function of Rad18 in germ cells of male mice. [Methods] Use germ line cell-specific marker TRA98 to detect the germ cells in the seminiferous tubules of 2-,6- and 12-month-old Rad18-/- mice. Use in situ hybridization to confirm the expression of Rad18 in the spermatogonia of 1-week wild type (WT) mice. [Results] The percentage of abnormal seminiferous tubules without spermatogonia in 12-month-old Rad18-/- testes was 39.5%，more than 2.76% in WT testes, P<0.05. The degenerated tubules in Rad18-/- testes retained normal Sertoli cells. No TRA98 positive germ cells entered the Rad18-/- epididymis. The spermatozoa found in the 12-month-old Rad18-/- epididymis displayed normal morphology. Rad18 was highly expressed in the spermatogonia of 1-week-old WT mice. [Conclusion] Rad18 is involved in maintaining spermatogonial stem cells. |
| Key words: Rad18-/-mice TRA98 spermatogonial stem cells |
