香菇C91-3菌丝发酵液蛋白口服液抗肿瘤免疫机制的探讨

Anti-tumor effect of proteins from lentinus edodes C91-3 mycelium′s fermentative liquid in vivo

关键词:  香菇发酵蛋白  抗肿瘤  IL-2  TNF-α

Key words:  lentinus edodes fermentative protein(LFP)  anti-tumor  IL-2  TNF-α

摘要:

[目的]探讨香菇C91-3菌丝发酵液蛋白（LFP91-3）口服液的抑制肿瘤作用及机制。[方法]取60只昆明小鼠，随机分6组后进行S180荷瘤，并对荷瘤小鼠分别给予蒸馏水和不同剂量的LFP91-3口服，观察剂量对小鼠生存期的影响，确定LFP91-3的最适剂量。同时观察口服最适剂量后荷瘤小鼠淋巴细胞增殖反应，血清IL-2和TNF-α含量的变化，以蒸馏水口服组为对照。[结果]LFP91-3口服液能延长荷瘤小鼠的生存期，且存在一定的剂量依赖关系。LFP91-3最适剂量组小鼠淋巴细胞增殖率，血清IL-2和TNF-α水平分别为（44.11±14.4）%，(48.33±16.7) pg/mL和(84.27±16.88) pg/mL，明显高于对照的蒸馏水组（18.04±8.72）%，（30.5±3.58）pg/mL和（35.05±17.29）pg/mL，P<0.05。[结论]LFP91-3口服液具有体内抑瘤作用，其抑瘤作用可能与机体免疫能力提高有关。

Abstract:

[Objective] The anti-tumor mechanism of oral protein liquid from C91-3 lentinus fermentative liquid (LFP91-3) was studied in this experiment.[Methods] Sixty mice in the S180 tumor models were randomly divieded into 6 groups and then were orally given LFP91-3  in different doses and water as control to research the effects of LFP91-3 liquid,on survival and find the optimal dose of the drug. Then lymphocyte proliferation, and serum content changes of IL-2 and TNF-α of the mice in the optimal dose group were observed in comparison to those in water group. [Results] Oral LFP91-3liquid could prolong the survival time of mice in the S180 tumor models, the spleen lymphocyte proliferation rate and serum IL-2 and TNF-α concentrations of the group with optimal dose of LFP91-3 were （44.11±14.4）%， (48.33±16.7) pg/mL， (84.27±16.88）pg/mL, respectively, being higher than those of group with water （18.04±8.72）%， (30.5±3.58) pg/mL， (35.05±17.29）pg/mL, (P<0.05).[Conclusion] Oral LFP91-3 liquid have potentiality to inhibit cancer, which maybe associate with immunity increasing effect of the drug.