小鼠腹水型肝癌高低转移细胞株DARS2蛋白表达的差异

Expression difference of DARS2 in two murine hepatocarcinoma strains with high and low metastatic potential

关键词:  DARS2蛋白  Hca-F细胞株  Hca-P细胞株  转移

Key words:  DARS2  Hca-F cell strain  Hca-P cell strain  metastasis

摘要:

[目的]研究具有高、低转移潜能的小鼠腹水型肝癌细胞株Hca-F与Hca-P中线粒体的天门冬氨酰转移核糖核酸合成酶（DARS2）蛋白表达的差异。[方法]将Hca-F细胞与Hca-P细胞分别接种于615小鼠腹腔，抽取腹水，培养后收集细胞；用免疫印迹分析及免疫细胞化学方法检测Hca-F与Hca-P细胞的DARS2蛋白表达。[结果]DARS2在高转移细胞株Hca-F与低转移细胞株Hca-P表达量分别为（0.219±0.016）与（0.473±0.043），二者比较差异有显著性意义 (P=0.01)。 [结论]DARS2蛋白表达降低可能与小鼠肝癌转移相关。

Abstract:

[Objective] To research the expression difference of DARS2 in two murine hepatocarcinoma strains（Hca-F and Hca-P）with high and low metastatic potentials. [Methods] The Hca-F cells and Hca-P cells were injected into abdominal cavity of 615 mice respectively, and then the ascitic cancer cells were drawn, cultured and collected. The protein expressions of DARS2 in the two strains were detected by western blot and immunocytochemistry. [Results] The protein expressions of DARS2 in Hca-F cells and Hca-P cells were 0.219±0.016 and 0.473±0.043, respectively. The difference was significant (P=0.01). [Conclusion] The decreasing expression of DARS2 protein may be related to metastasis potential of murine hepatocarcinoma.