核转录因子E2F-1、P27在大肠癌中的表达及意义

Expression and significance of nuclear transcription factor E2F-1 and P27 in colorectal adenocarcinoma

关键词:  大肠癌  E2F-1  P27  免疫组化

Key words:  colorectal adenocarcinoma  E2F-1  P27  immunohistochemisty

摘要:

[目的]探讨大肠癌组织E2F-1和P27表达的意义。[方法]用免疫组化SP法检测78例大肠癌、20例正常大肠黏膜、30例大肠腺瘤E2F-1和P27的表达情况，并分析二者在大肠癌发生中的相关性。[结果]E2F-1表达阳性率在大肠癌（55.13%）显著高于正常大肠黏膜（0%）及大肠腺瘤（6.67%）(P<0.05)；E2F-1表达与大肠癌的病理分级、淋巴结转移和临床分期相关 (P<0.05)。P27在正常大肠黏膜的阳性率（100%）显著高于大肠腺瘤（53.33%）和大肠癌（15.38%）(P<0.05)。P27表达与大肠癌的病理分级、淋巴结转移和临床分期相关 (P<0.05)。大肠癌中E2F-1与P27 表达呈负相关（r = -0.393，P<0.01）。[结论]E2F-1和P27表达与大肠癌发生关系密切，可作为大肠癌发生、发展的生物学标记物。

Abstract:

[Objective] To study the relationship of E2F-1 and P27 expression to occurence of colorectal adenocarcinoma and its clinical significance. [Methods] The expressions of E2F-1 and P27 were detected in 78 colorectal adenocarcinomas, 30 colorectal adenomas and 20 normal colorectal mucosa by immunohistochemical SP method. [Results] The positive expression rate of E2F-1 ( 55.13%) in the carcinoma was higher than those in colorectal adenoma (6.67%) and mucosa (0%) (P<0.05). The expression of E2F-1 in colorectal carcinoma was positively related to pathological grade, lymph node metastasis and clinical stage (P<0.05). P27 positive rate in normal colorectal mucosa (100%) was higher than those of colorectal adenoma (53.33%)and colorectal carcinoma (15.38%). The expression of P27 in colorectal carcinoma was negatively related to pathological grade, lymph node metastasis and clinical stage (P<0.05). In colorectal carcinoma, E2F-1 and P27 expressions were negatively correlated (r=-0.393, P<0.01). [Conclusion] E2F-1 and P27 is closely related to the colorectal adenocarcinoma occurrence, and both of them may have possibilities as biological markers in colorectal adenocarcinoma.