引用本文:郝帅,闫艾慧,姜学钧.顺铂体内注射对子代豚鼠耳蜗毛细胞的影响[J].大连医科大学学报,2011,33(5):434-436+440.
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顺铂体内注射对子代豚鼠耳蜗毛细胞的影响
郝帅, 闫艾慧, 姜学钧
中国医科大学 第一临床医院 耳鼻咽喉科,辽宁 沈阳 110001
摘要:
[目的]了解孕晚期顺铂暴露是否会诱导子代豚鼠耳蜗毛细胞损伤。[方法]将孕晚期豚鼠12只随机分为两组,在孕50 d至56 d,顺铂组经腹膜腔注射顺铂1.5 mg/kg·d,对照组注射生理盐水1.5 mg/kg·d。出生后14 d,应用原位末端转移酶标记技术(TUNEL)和Caspase-3免疫组化染色检测毛细胞凋亡情况。[结果]孕晚期顺铂暴露的子代豚鼠耳蜗毛细胞有凋亡发生及Caspase-3蛋白表达。[结论]孕期顺铂暴露可诱导子代豚鼠耳蜗毛细胞Caspase-3表达及凋亡发生。
关键词:  毛细胞  凋亡  顺铂
DOI:10.11724/jdmu.2011.05.05
分类号:R764.35
基金项目:
Effect of cisplatin-injected on cochlear hair ceus in newborn guinea pigs
HAO Shuai, YAN Ai-hui, JIANG Xue-jun
Department of Otolaryngology,the First Affiliated Hospital,China Medical University,Shenyang 110001,China
Abstract:
[Objective]To evaluate the role of pregnant cisplatin exposure in inducing apoptosis on the development of cochlear in newborn guinea pigs.[ Methods]On gestational day 50 to 56,pregnant guinea pigs were intraperitoneally injected with 1.5 mg/kg body weight cisplatin and normal saline once a day,respectively.On postnatal day 14,the temporal bones of guinea pig pups were removed, immunohistochemistry was used for detecting the expression of caspase-3 and TUNEL method for hair cell apoptosis.[Results]Apoptosis and caspase-3 expression were observed in cochlear hair cells of newborn guinea pigs with pregnant cisplatin exposure.[Conclusion]Perinatal cisplatin exposure would induce cochlear hair cell apoptosis.
Key words:  hair cell  apoptosis  pregnant cisplatin exposure