| 摘要: |
| ［目的］探讨丁香胃灵合剂(CWLM)对慢性萎缩性胃炎（CAG）大鼠血清生长抑素（somatostatin，SS）及胃泌素（gastrin，Gas），胃黏膜Bax的影响。［方法］将健康Wistar大鼠随机分为5组，即正常组、CAG组、CWLM低、中、高剂量组，每组12只。用水杨酸钠灌胃联合自由饮用氨水法建立大鼠CAG模型，正常组予生理盐水灌胃。造模成功后开始给予药物干预，CWLM按16 g/kg·d、8 g/kg·d、4 g/kg·d的剂量给大鼠连续50 d灌服，取材后采用ELISA法检测血清SS、Gas的含量，免疫组化法检测胃黏膜Bax的表达变化。［结果］与正常组比较，CAG组SS含量显著升高（P<0.01），Gas含量显著降低（P<0.01），胃黏膜Bax表达显著降低（P<0.01）；与CAG组比较，CWLM（高、中剂量）组SS含量明显下降，Gas含量显著升高，胃黏膜Bax表达明显升高（P<0.01或P<0.05），其中高剂量组差异最为突出（P<0.01）。
［结论］CWLM对CAG模型大鼠有较好的治疗作用，这一作用可能与其减少血清SS含量，升高血清Gas含量及上调胃黏膜Bax表达有关。 |
| 关键词: 丁香胃灵合剂 慢性萎缩性胃炎 SS Gas Bax |
| DOI：10.11724/jdmu.2011.04.06 |
| 分类号:R289 |
| 基金项目: |
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| Effect of clove wei ling mixture on the quantities of gastrin and somatostatin in serum and expression of bax of mucosa in rats with chronic atrophic gastritis |
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LOU Hua1, ZHU Yao-huan2, LI Hui-peng1, LI Su-jie3
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1.Postgraduate Student of 2008,Dalian Medical University,Dalian 116044,China;2.Department of Chinese Medicine,the Second Affiliated Hospital of Dalian Medical University,Dalian 116027,China;3.The School Physician Room,Dalian Institute for Nationalities,Dalian 116600,China
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| Abstract: |
| ［Objective］To observe the protective effects of clove wei ling mixture (CWLM) on the mucosa of chronic atrophic gastritis in rats. ［Methods］Sixty Wistar rats were randomly divided into normal control group, chronic atrophic gastritis (CAG) group,CWLM low-dose group, CWLM middle-dose group and CWLM high-dose Group. CAG model was established in Wistar rats by synthetical method:sodium salicylate was given every day orally, free drink ammonia, with irregular diet animal model for 8 weeks, and the normal control rats were given normal saline. Drug intervention began after being made model 2 months, CAG rats were treated with CWLM 16g/kg d, 8g/kg d and 4g/kg d, respectively. After 50 days, all the rats were anesthetized and the levels of Gas and SS in sera were measured with ELISA and Bax expression in gastric mucosa was detected with immunohistochemistry. ［Results］Compared to normal control group, the level of SS in the CAG group was significantly higher,but the level of Gas and the expression of Bax were significantly lower (P<0.01). Compared to CAG group, the level of SS was lower, but the level of Gas and the expression of Bax in three treatment groups were higher significantly (P<0.01 or P<0.05). ［Conclusion］CWLM has beneficial effect for treatment of CAG in rats, which maybe related to increasing of Gas and Bax with decreasing of SS. |
| Key words: Clove wei ling mixture chronic atrophic gastritis (CAG) SS Gas Bax |
