宫颈癌组织中Rap1GAP蛋白表达及其与HPV感染的相关性

Rap1GAP protein expression in cervical carcinoma and its relationship to HPV infection

关键词:  宫颈癌  Rap1GAP  HPV

Key words:  cervical carcinoma  Rap1GAP  HPV

摘要:

［目的］探讨Rap1GAP蛋白在宫颈癌组织中的表达变化以及其与HPV感染之间的关系。［方法］免疫组化方法检测20例宫颈癌与20例正常宫颈组织 HPV感染和Rap1GAP蛋白表达情况。 ［结果］HPV检出阳性率在正常宫颈组织和宫颈癌中分别为20%与75%，宫颈癌组明显高于正常宫颈组（P<0.05）。宫颈癌组的Rap1GAP蛋白表达阳性率为15%、弱阳性率为15%，二者均明显低于正常宫颈组（阳性率，55%和弱阳性率，45%）（P均<0.05）。宫颈癌组织中，HPV阳性组Rap1GAP 蛋白缺失率为80%，明显高于HPV阴性组的缺失率40%（P<0.05），且二者之间呈正相关（r=0.553，P<0.05）。［结论］宫颈癌组织Rap1GAP蛋白表达下调或缺失可能与HPV感染有关。

Abstract:

［Objective］To reveal the change of Rap1GAP protein expression in cervical carcinoma and its relationship to HPV infection.［Methods］HPV and the expression of Rap1GAP protein in 20 samples of normal cervix and 20 samples of cervical carcinoma were detected by immunohistochemistry.［Results］The HPV infection rate was 75% in cervical carcinoma,higher than that of 20% in normal cervix (P<0.05).The positive and weak positive expression rates of Rap1GAP protein were 15% and 15% in cervical carcinoma,significantly lower than those of 55% (P<0.05) and 45% (P<0.05) in normal cervix tissues.In cervical carcinoma,deletion rate of Rap1GAP protein expression in HPV-positive samples was 80%,significantly higher than that of 40% in HPV-negative (P<0.05）and there was a positive correlation between the deletion of Rap1GAP protein and HPV infection (r=0.553,P<0.05).［Conclusion］Expression of Rap1GAP protein is down-regulated or deleted in cervical cancer and it has a positive correlation with HPV infection, implying that there is a relationship among HPV infection, Rap1GAP protein expression deletion and cervical cancer.