雷公藤多苷致小鼠卵巢早衰后的自然修复时间探讨

Triptergium wifordii induced premature ovarian failure after the natural rehabilitation

关键词:  雷公藤多苷  卵巢早衰  自然修复  小鼠

Key words:  Triptergium wilfordii  premature ovarian failure  premature ovarian failure  mice

摘要:

［目的］考察雷公藤多苷造成小鼠卵巢早衰停药后能否自然修复及修复周期。［方法］取健康小鼠25只，采用随机数字表方法取出5只作为正常对照组，其余作为模型对照组，实验小鼠灌胃给雷公藤多苷15 d后，通过外观观察；血清E2、P检测；阴道脱落细胞涂片；子宫、卵巢指数测定等，判断雷公藤多苷对小鼠卵巢的影响及停药后的自然修复情况。［结果］雷公藤多苷片40 mg·kg-1/d灌胃，连续15 d能造成确切的雌性小鼠生殖功能低下状态，具体表现为，模型对照组与正常对照组比较，动情周期出现紊乱，血清雌二醇E2水平明显降低，与对照组相比，差异有显著性意义（P<0.05）；孕酮P较正常对照组有降低趋势，但差异无显著性意义（P>0.05）；子宫指数、卵巢指数均明显降低（P<0.05）。停药10 d后，模型组小鼠生殖功能呈自行恢复趋势，动情周期、性激素水平基本恢复正常。［结论］雷公藤多苷口服连续灌胃15 d 后可造成小鼠卵巢早衰，停药10 d后，卵巢功能即可自然修复。

Abstract:

［Objective］To investigate the process of premature ovarian failure after Triptergium wilfordii (TW) treatment,whether the ovarian function in POF mice can natural recovery or not after the ending of treatment.［Methods］After 15 days of TW treatment,we detected the serum level of E2 and P,and the indexes of uterus and ovarian.We also documented the smear of vaginal exfoliated cells.［Results］All mice appeared poor status of physiological function.The cycle of estrus in the model group became disorder as compared with that of the control group and levels of E2 in the model group were significantly decreased than that in the control group (P<0.05).However,levels of P in the model group were only changed slightly when compared with that of the control group (P>0.05).Uterus index and ovarian index were also significantly lower in the model group (P<0.05).After ending of treatment,the ovarian function in POF mice could spontaneously recover and estrous cycle and estrogen levels returned to normal within 10 days.［Conclusion］Triptergium wifordii can cause premature ovarian failure and the ovarian function in POF mice can gradually recover 10 days later.