缺氧诱导因子-1α及血管内皮生长因子在卵巢上皮性肿瘤中的表达

Expression of hypoxia-inducible factor 1α and vessel endotriul growth factor in ovrarian cancer

关键词:  卵巢癌  缺氧诱导因子-1α  血管内皮生长因子

Key words:  ovarian cancer  hypoxia-inducible factor 1α  vessel endotriul growth factor

摘要:

［目的］ 探讨缺氧诱导因子-1α（HIF-1α）及血管内皮生长因子（VEGF）在卵巢肿瘤组织中的表达。［方法］ 选取卵巢癌组织58例、交界性肿瘤23例作为实验组，正常卵巢组织38例作为对照组。应用免疫组化SP法和蛋白印迹免疫分析法检测三者HIF-1α及VEGF表达。［结果］ HIF-1α在卵巢癌、交界性肿瘤和正常卵巢阳性表达率分别为86.21%、47.83%和13.16%，三者比较差异均有显著性意义；HIF-1α的表达与临床分期正相关（P<0.05），且随病理分级增加而增强（P<0.05）；VEGF在三种组织均有表达，其阳性表达率分别为98.28%、82.61%和57.89%，两两比较差异均有显著性意义（P<0.05）；VEGF表达与卵巢癌临床分期正相关；且随病理分级增加而增强（P<0.05）。蛋白印迹免疫分析显示卵巢癌组织HIF-1α及VEGF蛋白表达量分别是正常卵巢组织的4.58±1.73及4.32±1.55倍。［结论］ 检测卵巢癌组织中HIF-1α及VEGF有助于卵巢癌的早期诊断、预后判断。

Abstract:

［Objective］ To investigate the expression of HIF-1α and VEGF in ovarian cancer tissue.［Methods］ The expression of HIF-1α and VEGF were assessed by imunohistochemical staining and western-blot method in 58 cases of ovarian cancer, 23 cases of carcinoma in situ and 38 cases of nomal ovarian tissue. ［Rusults］ Immunohistochemical staining:HIF-1α level in ovarian carcinoma was much higer than those in carcinoma in situ and nomal tissue, their positive staining rate were 86.21%, 47.83% and 13.16% (P<0.05). The expression of HIF-1α elevatated as the clinical stage increased (P<0.05) and the pathologic grade increased (P<0.05). VEGF level in ovarian carcinoma was higer than those in carcinoma in situ and nomal tissue, their positive staining rate was 98.28%, 82.61%, and 57.89% (P<0.05). The expression of VEGF elevatated as the clinical stage increased (P<0.05) and the pathologic grade increased (P<0.05). VEGF has positive correlation with HIF-1α. Western-blot: the protein band of HIF-1α and VEGF is at 120 kD and 23 kD, the OD of HIF-1α is 4.58±1.73 times while VEGF is 4.32±1.55 times in early invasive stage as that in normal tissue. ［Conclusions］ The expression of HIF-1α and VEGF can be used to accurately assess the prognosis in ovarian cancer, and may provide new therapy target.