| 引用本文: | 王雪娇,张开立,王宏宇,胡先福,李世杰,王 茜,李 宏,孙 媛.DNMT1与UHRF1基因在胃癌细胞系中的表达特点及其与癌细胞DNA甲基化的关系[J].大连医科大学学报,2010,32(1):50-53. |
| |
|
| 摘要: |
| ［目的］探讨DNMT1和UHRF1在胃癌的形成和转化中的表达特点和可能的生物学作用。［方法］ 提取CIMP型H（High）的胃癌细胞系HGC-27和CIMP型为L（low）的胃癌细胞系MGC803，BGC823及AGS的核酸样品进行UHRF1和DNMT1表达的分析。［结果］ DNMT1在MGC823和HGC-27表达水平明显高于其他胃癌细胞系（P<0.01）；UHRF1在HGC-27中有表达水平高于其他3个胃癌细胞系（P<0.01）。［结论］胃癌细胞DNA的甲基化不但和DNMT1有关而且和UHRF1也有着密切关系。 |
| 关键词: 胃癌 UHRF1 DNA甲基化 CIMP型 DNMT1 |
| DOI：10.11724/jdmu.2010.01.12 |
| 分类号:R734 |
| 基金项目: |
|
| DNMT1 and UHRF1 expression patterns in gastric cancer cells and their correlation with DNA methylation |
|
WANG Xue-jiao1, ZHANG Kai-li2, WANG Hong-yu1, HU Xian-fu1, LI Shi-jie1, WANG Qian2, LI Hong2, SUN Yuan2
|
|
1.Faculty of Clinical Medicine, College of Basic Medical Sciences;2.Liaoning Laboratory of Cancer Genomics and Department of Cell Biology, Dalian Medical University, Dalian 116044, China
|
| Abstract: |
| ［Objective］ To elucidate DNMT1 and UHRF1 expression patterns of gastric cancers and their potential roles in gastrocarcinogenesis. ［Methods］ Samples of RNAs were isolated from established human gastric cancer cell lines such as MGC803, BGC823 and AGS with CIMP-low and HGC-27 with CIMP-high DNA methylation patterns, and UHRF1 and DNMT1 expression was analyzed by RT-PCR. ［Results］ DNMT1 was up-regulated in MGC823 and HGC cells, while it was expressed in low level in MGC803 and HGC-27 (P<0.01). UHRF1 expression level in CIMP-H HGC-27 cells was higher than that in the other three cell lines (CIMP-L), P<0.01. ［Conclusion］ DNA methylation in gastric cancer cells is correlated either with DNMT1 or with UHRF1 expression. |
| Key words: gastric cancer UHRF1 DNA methylation CIMP type DNMT1 |
