| 摘要: |
| ［目的］了解临床分离的耐亚胺培南铜绿假单胞菌（PAE）的相关耐药基因存在状况。［方法］ 对16株铜绿假单胞菌采用PCR检测亚胺培南耐药的相关基因（TEM、OXA-10群、SHV、oprD2、IMP、CARB、blaDHA、VIM、PER、GES、VEB）。［结果］ 16株铜绿假单胞菌TEM阳性4株（25.00%）、OXA-10群阳性5株（31.25%）、SHV阳性5株（31.25%）、orpD2阳性16株（100%）、IMP阳性2株（12.50%）CARB阳性1株（6.30%）、blaDHA阳性1株（6.30%）、VIM阳性1株（6.30%）、PER阳性0株（0）、GES阳性1株（6.30%）、VEB阳性0株（0），且部分菌株携带3个以上的耐药基因。［结论］ 临床分离的耐亚胺培南铜绿假单胞菌携带多种耐药基因，没有导致院内感染的流行耐药菌株。 |
| 关键词: 铜绿假单胞菌 亚胺培南 耐药性 基因 |
| DOI：10.11724/jdmu.2009.05.21 |
| 分类号:R466.1 |
| 基金项目: |
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| Genes for Imipenem antibiotic resistance in Pseudomonas aeruginosa |
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WANG Jing, HAN Li-xia
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Department of Clinical Laboratory, the First Affiliated Hospital of Dalian Medical University, Dalian 116011, China
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| Abstract: |
| ［Objective］ To investigate genes associated with the drug-resistance of Imipenem antibiotics in Pseudomonas aeruginosa (PAE) isolated from clinical patients. ［Methods］ Genes including TEM, SHV, OXA-10, IMP, oprD2, CARB, blaDHA, VIM, PER, GES and VEB were detected by PCR amplification in 16 PAE isolates. ［Results］ TEM, SHV, IMP, OXA-10, oprD2, CARB, blaDHA, VIM, PER, GES and VEB genes were positive in 25.00%, 31.25%, 31.25%, 12.50%, 100%, 6.30%, 6.30%, 6.30%, 0, 6.30% and 0 of 16 isolates. Some of the strains carried more than 3 resistance genes. ［Conclusion］ Pseudomonas aeruginosa carries various and more than one beta-lactamase genes in clinical Patients. |
| Key words: Pseudomonas aeruginosa Imipenem drug susceptibility gene |
