实时荧光PCR方法对乙肝病毒基因分型的检测及临床意义

Detection of hepatitis B virus gene type with RT-PCR method and its clinical significance

关键词:  乙肝病毒  基因分型  临床意义

Key words:  hepatitis B virus  genotype  clinical significance

摘要:

［目的］ 了解乙肝患者的HBV基因分型特点及不同乙型肝炎病毒基因型对肝脏的损害程度，了解不同乙肝基因型的临床用药效果。［方法］ 随机选取乙型肝炎患者血清100例，已确诊的慢性乙肝血清50例，肝硬化患者的血清20例和追踪治疗的乙肝患者血清15例。用实时荧光PCR（RT-PCR）方法对其进行基因分型的检测。［结果］ 在100例乙肝患者中，B型20例，占20%；C型71例，占71%；BC混合型1例，占1%；非B非C型8例，占8%。肝硬化患者中，以C型为主。［结论］ 乙肝基因型主要以C型为主，B基因型与C基因型的临床治疗效果比其他基因型差，且C基因型感染的患者肝硬化发生率高。

Abstract:

［Objective］ To understand the character of hepatitis B virus genotype, know the damage of liver in different hepatitis B virus genotype infection, and observe the curative effects of medications on different hepatitis B virus genotype infection. ［Methods］ Random choose serums from 100 patients with HBV infection, 50 patients diagnosed as chronic hepatitis B, 20 patients with liver cirrhosis and 15 hepatitis B patients that have been treated. Their genotype was detected with real-time PCR method. ［Results］ In 100 samples with HBV infection, 20% of them were genotype B, 71% were genotype C, 1% was mixed type, and 8% of the samples were non-B and non-C. Genotype C was predominant genotype in liver cirrhosis. ［Conclusion］ Genotype C is predominant genotype. The treatment result in genotype B and genotype C is worse than that in other types. The incidence rate of liver cirrhosis is high in genotype C infection.