摘要: |
[目的]建立高效液相色谱法(HPLC法)测定硝苯地平片中硝苯地平含量的方法,为开展血中药物浓度测定及临床合理用药提供依据。[方法]以甲醇-水(3∶2)为流动相,流速为1.0 mL·min-1,检测波长235 nm。[结果]硝苯地平浓度在6.132~14.308 μg·mL-1范围内线性关系良好。相关系数r为0.9999,平均相对回收率为99.98%,日内RSD为0.70% ;日间RSD为1.25%。[结论]本法适用于硝苯地平片含量的测定。 |
关键词: 硝苯地平片 高效液相色谱法 紫外分光光度法 |
DOI:10.11724/jdmu.2008.04.13 |
分类号:R91 |
基金项目: |
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Determination of content of Nifedipine in tablets by HPLC |
GAO Yan1, QIU Yi-lan2, DONG Li2
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1.Dalian Shahekou District Hospital;2.Institute for Drug Control in Dalian Prefecture, Dalian 116021,China
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Abstract: |
[Objective]To establish a HPLC method for the determination of the content of Nifedipine in the tablets.[Methods]For the detection, the mobile phase was methanol-water (3∶2), the flow rate was 1.0 mL·min-1 and the wavelength of HPLC and UV detection was 235 nm and 333 nm.[Results]The linearity was obtained in the rang of 6.132~14.308 μg·mL-1 of Nifedipine (r =0.9999). The average recovery was 99.98%, the relative standard deviation of inter-day and intra-day was 0.70% and 1.25%, respectively.[Conclusions]The established HPLC method can be applied for the determination of content of Nifedipine in the tablets ,which are helpful for clinical medication. |
Key words: Nifedipine Tablets HPLC UV |