PUVA对HL-60细胞凋亡及线粒体膜电位的影响

Effects of PUVA on apoptosis and mitochondrial membrane potential in HL-60 cells

关键词:  PUVA  白血病  HL-60细胞  凋亡  线粒体膜电位

Key words:  PUVA  leukemia  HL-60 cells  apoptosis  mitochondrial membrane potential PSO

摘要:

［目的］研究补骨脂素（PSO）加长波紫外线（UVA）光化学疗法（PUVA）对人白血病细胞HL-60凋亡及线粒体膜电位的影响。［方法］HL-60细胞接受或不接受UVA照射后，在不同浓度PSO培养，电镜下观察细胞超微结构改变，流式细胞仪（FCM）检测细胞凋亡率和线粒体膜电位变化，多因素方差分析法进行统计学处理。［结果］PUVA处理后的HL-60细胞超微结构出现明显的凋亡形态；PSO、UVA照射及PUVA在作用后24 h均可使细胞凋亡率增加，ΔΨm下降，对照组、80 μg/mL PSO组、5 min UVA组及PUVA（80 μg/mL PSO+5 min UVA）组凋亡率分别为（10.60±0.31）%、（26.94±0.26）%、（28.53±0.05）%、（37.72±0.09）%；线粒体膜电位水平分别为388.47±6.35、173.17±10.89、200.00±2.91以及71.83±9.47,各组间 P<0.01，PUVA的作用明显强于前两者。［结论］PUVA可诱导人白血病细胞HL-60发生凋亡，诱导凋亡的途径之一为下调线粒体膜电位。

Abstract:

［Objective］To study the effects of psoralen(PSO) and long wave ultraviolet light(PUVA) on apoptosis and mitochondrial membrane potential in HL-60 cells.［Methods］HL-60 cells were incubated with PSO in different concentrations irradiated with or without UVA. The changes of ultrastructure of cells were observed under the electron microscope. The apoptosis ratios and the mitochondrial membrane potential were detected through the flow cytometry. The factorial design and analysis of variance were used to analyze the interaction among the factors.［Results］There were obvious ultrastructure changes about apoptosis in HL-60cells after treated with PUVA. PSO, UVA and PUVA all increased the apoptosis ratios and decreased the mitochondrial membrane potential. The apoptosis ratios of control group, 80 μg/mL PSO group, 5 min UVA group and PUVA (80 μg/mL PSO+5 min UVA) group were (10.60±0.31)%,(26.94±0.26)%,(28.53±0.05)% and (37.72±0.09)% respectively. The level of mitochondrial membrane potential of the four groups were 388.47±6.35, 173.17±10.89, 200.00±2.91 and 71.83±9.47 respectively (P<0.01) and the effects of PUVA were stronger than the other two.［Conclusions］PUVA can induce the apoptosis of HL-60 cells and one of the pathway to induce apoptosis is to downregulate the mitochondrial membrane potential.