| 摘要: |
| ［目的］探讨AQP9水通道蛋白在大鼠前列腺组织中的表达以及雄激素对前列腺AQP9表达的影响。［方法］使用20只8周龄去势大鼠模型，应用定量PCR以及免疫组织化学染色方法检测AQP 9 mRNA和蛋白在大鼠前列腺组织中的表达以及雄激素对大鼠前列腺AQP9表达的影响。［结果］正常大鼠前列腺组织中AQP9 mRNA和蛋白均有所表达（AQP9/β-actin 2.259±0.453），去势可减少大鼠前列腺中AQP9的表达（0.422±0.048），睾酮注射可恢复AQP9在前列腺组织中的表达（2.098±0.507）。［结论］大鼠前列腺组织中AQP9的表达受雄激素的调控。 |
| 关键词: AQP 前列腺 雄激素 |
| DOI：10.11724/jdmu.2008.03.09 |
| 分类号:Q57 |
| 基金项目: |
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| Expression of aquaporin 9 in rat prostate is regulated by androgen |
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WANG Jian-bo1,2, SONG Xi-shuang1,2, NOZOMU Tanji3, YOKOYAMA Masayoshi3
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1.Department of Urology, the First Affiliated Hospital of Dalian Medical University, Dalian, 116011 China;2.;3.Department of Urology, Ehime University School of Medicine, Ehime, Japan
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| Abstract: |
| ［Objective］To study the expression of aquaporin 9(AQP9) in rat prostate and the regulation between AQP9 and androgen.［Methods］Twenty 8-weeks-old Sprague-Dawley(SD) rats were used as animal model in this study, the regulation between AQP9 and androgens was examined using real-time PCR and immunohistochemistry.［Results］AQP9 mRNA and protein were expressed in rat prostate(AQP9/β-actin 2.259±0.453), surgical castration decreased the expression(0.422±0.048)and testosteron injection restored it(2.098±0.507).［Conclusions］The expression of AQP9 in rat prostate is modulated by androgen. |
| Key words: Aquaporin prostate androgen |
