Bcl-2基因在口腔上皮癌多药耐药细胞的表达

Expression of Bcl-2 in KBV cell

关键词:  KBV/ KB  Bcl-2  多药耐药性

Key words:  KB  KBV  Bcl-2  multidrug resistance

摘要:

［目的］通过检测Bcl-2基因在口腔上皮癌敏感细胞株（KB）与多药耐药细胞株（KBV）的表达差异，探讨Bcl-2基因在口腔上皮癌细胞多药耐药性发生中的作用。［方法］体外培养口腔上皮癌多药耐药株及敏感株，通过RT-PCR法及免疫荧光法检测Bcl-2基因在KB及KBV细胞中转录及翻译水平的差异，通过MTT法及流式细胞检测技术检测KB及KBV细胞对长春新碱（VCR）敏感度的差异。［结果］免疫荧光图像显示Bcl-2蛋白在KB细胞中弱表达，而在KBV细胞中强表达；RT-PCR检测结果显示，KB细胞Bcl-2/ β-actin比值是25.0%，KBV组Bcl-2/ β-actin比值为36.1%，两组细胞间差异具有统计学意义（P<0.01）；MTT法检测结果显示KBV组细胞对长春新碱的耐药性为KB组的16.5倍，流式细胞仪检测结果显示KB细胞凋亡率达（98.8±1.2）%；KBV细胞凋亡率达（23.5±2.1）%，两组间差异具有统计学意义（P<0.01）。［结论］Bcl-2基因与口腔上皮癌耐药性的发生密切相关。

Abstract:

［Objective］To investigate the effect of Bcl-2 on development of the multidrug resistance in KBV cells.［Methods］RT-PCR and immunofluorescent method were used to detect the Bcl-2 expression at the mRNA and protein levels in KB and KBV cells. MTT and flow cytometry(FCM) were used to detect the chemosensitivity to VCR in KB and KBV cells.［Results］The immunofluorescent image in KBV cells was stronger than that in KB cells at the Bcl-2 protein level. At the mRNA level, Bcl-2/β-actin was 36.1% in KBV cells and 25.0% in KB cells , P<0.01.The apoptosis rates in KB and KBV cells were (98.8±1.2)% and cells (23.5±2.1)% respectively, P<0.01.［Conclusions］This result suggested that Bcl-2 might play an important role in development of multidrug resistance of KBV cells.