胃癌及癌前病变中Survivin和 COX-2蛋白的表达及其与Hp感染的关系

Expressions of Survivin，COX-2 in GPL and gastric carcinoma and their association with helicobactor pylori infection

关键词:  生存素  COX-2  幽门螺杆菌  胃癌  胃癌前病变

Key words:  Survivin  COX-2  Hp  gastric carcinoma  gastric precancerous lesions

摘要:

［目的］分析胃癌及癌前病变survivin、COX-2蛋白表达与幽门螺杆菌（Helicobacter pylori，H pylori）感染的关系，探讨胃癌发病的可能机制。［方法］收集胃癌标本51例，非癌胃黏膜标本47例，应用快速尿素酶试验与W-S 银染色法将各种病变分为Hp感染阳性组和阴性组，应用免疫组化SP法对各种病变survivin、COX-2蛋白的表达进行检测。采用χ² 检验进行数据分析。［结果］Survivin蛋白的表达从慢性浅表性胃炎(CSG)到胃癌(GC)逐渐增加，其中慢性萎缩性胃炎(CAG) 组明显低于肠化生(IM)、不典型增生(Dys)和胃癌(GC)组（Survivin 30.5% vs 60.0%、62.5%、70.6%，P＜0.05），而IM、Dys组与GC组差异无显著性意义（P>0.05）；COX-2蛋白在CSG、CAG、IM、Dys、GC中的表达率分别为0%、23.1%、60.0%、50.0%、68.6%。其中，CAG组亦明显低于IM、Dys、GC组（P＜0.05），而IM、Dys组与GC组差异无显著性意义（P>0.05）。在GC组，Hp阳性者Survivin、COX-2表达明显高于Hp阴性者（Survivin 70.6% vs 29.4%，COX-2 68.6% vs 31.4%，P＜0.01）。［结论］Survivin、COX- 2 可能参与胃癌的早期形成, Hp 感染可能是其过度表达的机制之一。

Abstract:

［Objective］To observe expressions of survivin and COX-2 in gastric carcinoma and gastric precancerous lesion(GPL) and further elucidate the possible effect of Helicobacter pylori(H pylori) infection on gastric carcinoma pathogenesis.［Methods］Collect gatric carcinoma samples 51 cases and non-cancer gastric mucosa 47 cases.Rapid urease test and Warthin-Starry method were used for examing H pylori infection of these patients. The expressions of survivin and COX-2 were examined by immunohistochemical SP technique in gastric epithelial cells．Chi-square was used for data analysis.［Results］Of all patients,the expression of survivin was increased from CSG to GC, the positive rate of survivin in CAG was significantly lower than that in IM,Dys,GC(Survivin 30.5% vs 60.0%,62.5%,70.6%,P＜0.05), but there was no difference among IM, Dys and GC(P>0.05);The positive rates of COX-2 were 0%,23.1%,60.0%,50.0%,68.6% respectively in CSG,CAG,IM,Dys and GC,the positive rate of COX-2 in CAG was significantly lower than that in IM,Dys,GC(P＜0.05), but there was no difference among IM, Dys and GC(P>0.05);In Hp positive GC group the expressions of survivin and COX-2 were higher than Hp negative GC group(Survivin 70.6% vs 29.4%,COX-2 68.6% vs 31.4%,P＜0.01).［Conclusion］Survivin and COX-2 protein overexpressions may contribute to an early event of gastric cancer development and Hp infection may be one of the mechanisms of survivin,COX-2 overexpression.