乳腺癌细胞中COX-2、MMP-9蛋白表达与其淋巴道转移的关系

Relationship between expressions of COX-2, MMP-9 in breast carcinoma and lymph node metastasis

关键词:  乳腺癌  淋巴道转移  环氧化酶-2  基质金属蛋白酶-9

Key words:  breast carcinoma  lymph node metastasis  cyclooxygenase-2  matrix metalloproteinase-9

摘要:

［目的］探讨环氧化酶-2（COX-2）和基质金属蛋白酶-9（MMP-9）与乳腺癌浸润和转移的关系。［方法］采用免疫组织化学SP法检测74例乳腺浸润癌（有淋巴结转移者39例，无淋巴结转移者35例）中COX-2和MMP-9蛋白的表达情况，并用多因素Cox比例风险模型分析患者的预后。［结果］COX-2和MMP-9蛋白表达在淋巴结转移组乳腺癌肿瘤细胞中的平均秩次分别为42.90和44.58，在无淋巴结转移组乳腺癌肿瘤细胞中的平均秩次分别为31.49和29.61，二者在淋巴结转移组与无转移组的表达差异均具有显著性意义（P＜0.05），与乳腺癌淋巴结转移呈正相关。COX-2蛋白的表达随着乳腺癌病理分级的增高而增强。［结论］乳腺癌细胞中COX-2和MMP-9蛋白表达与其淋巴道转移相关，检测这两种蛋白表达将有助于判断乳腺癌的转移潜能及预后。

Abstract:

［Objective］To investigate the associations of cyclooxygenase-2(COX-2) and matrix metalloproteinase-9 (MMP-9) with invasion and metastasis of breast carcinoma.［Methods］The expressions of COX-2 and MMP-9 were examined in 74 cases of breast carcinoma including 39 cases with lymph node metastasis and 35 cases without lymph node metastasis by immunohistochemistry. The patient's prognosis was analyzed by Multivariable Cox proportional hazards model.［Results］The average ranks of COX-2 and MMP-9 were 42.90 and 44.58 in lymph node metastasis group, and 31.49 and 29.61in no lymph node metastasis group, both with significant difference (P＜0.05), and these expressions were positively correlated with the lymph node metastasis of breast carcinoma. The expression of COX-2 was related with the tissue grade of breast carcinoma (P＜0.05).［Conclusion］The expressions of COX-2 and MMP-9 are strongly associated with lymph node metastasis of breast carcinoma. These proteins are indicators of metastasis and predictors for prognosis of breast carcinoma.