胃癌Survivin 、COX-2 蛋白的表达及其相关性分析

Expression of survivin and COX-2 in gastric carcinoma and their relationship

关键词:  survivin  COX-2  胃癌  免疫组织化学  TUNEL  凋亡  凋亡指数

Key words:  COX-2  gastric carcinoma  immunohistochemistry  TUNEL  apoptosis  apoptosis index

摘要:

［目的］探讨胃癌中Survivin蛋白表达与胃癌生物学行为及凋亡相关蛋白COX-2和凋亡指数（AI）的相关性。［方法］ 应用免疫组化SP法检测51例胃癌组织、47例非癌胃黏膜组织中Survivin 和 COX-2的表达，应用TUNEL法检测 AI 。［结果］Survivin蛋白在胃癌组织中的阳性表达率为70.58 %较非癌胃黏膜组的36.17％明显增高（P<0.05），COX-2 在胃癌组织中的阳性表达率为68.63 %较非癌胃黏膜组的30.04％明显增高（P<0.05）。 Survivin蛋白在有淋巴结转移组阳性表达率显著高于无淋巴结转移组 （94.59% vs 28.57%，P<0.05），侵及浆膜组阳性表达率明显高于未及浆膜组（78.04% vs 40.00%，P<0.05），且在Ⅲ~Ⅳ期胃癌病人Survivin阳性表达率明显高于Ⅰ~Ⅱ期（88.57% vs31.25%，P<0.05），COX-2蛋白在淋巴结转移组阳性表达率明显高于无淋巴结转移组（78.38% vs 28.57%，P<0.05），且Ⅲ~Ⅳ期病人阳性表达率明显高于Ⅰ~Ⅱ期（77.14% vs 37.50%，P<0.05）。Survivin阳性表达与COX-2阳性表达呈正相关（r_s=0.32，P<0.05）。Survivin阳性组的平均 AI 明显低于阴性组（P<0.05），而COX-2阳性组的平均 AI明显高于阴性组（P<0.05）。［结论］Survivin 和COX-2蛋白与胃癌的发生及其恶性演变有关，检测Survivin 和COX-2蛋白有利于判断胃癌的恶性程度和预后。

Abstract:

［Objective］To observe the relationship of survivin expression to the biological behavior,the expression of COX-2 and apoptosis index（AI）in gastric carcinoma．［Methods］The expressions of survivin and COX-2 were examined in 51 cases of the gastric carcinoma and 47 cases of peri-carcinoma tissues by immunohistochemical SP and TUNEL technique．［Results］The positive rate of survivin in gastric carcinomas being 70.58% was significantly higher than that in peri-carcinoma tissues being 36.17％（P<0.05）; the positive rate of COX-2 in gastric carcinomas being 68.63% was significantly higher than that in peri-carcinoma tissues being 30.04%（P<0.05）．The positive rates of survivin in the groups with serosal invasion , with lymph node metastases and in TNM stage Ⅲ~Ⅳ being78.04%, 94.59% and 88.57% respectively were siginifcantly higher than those in the groups without serosal invasion , without lymph node metastasis and in TNM stage Ⅰ~Ⅱbeing 40.00%, 28.57% and 31.25% （P<0.05; P<0.05; P<0.05）．The positive rates of COX-2 in the groups with lymph node metastases and TNM stage Ⅲ~Ⅳ being 78.38% and 77.14% were siginifcantly higher than those in the groups without lymph node metastasis and in TNM stageⅠ~Ⅱbeing 28.57% and 37.50% （P<0.05; P<0.05）．The expression of survivin was positively correlated with expression of COX-2（r_s=0.32,P<0.05）．The mean AI in survivin-positive tumors were significantly lower than that in survivin-negative tumors（P<0.05），the mean AI in COX-2-positive tumors were significantly higher than that in COX-2- negative tumors（P<0.05）．［Conclusions］Survivin and COX-2 were associated with the oncogenesis and malignant progression of gastric carcinoma．Effective detection of survivin and COX-2 proteins may contribute to the evaluation of malignancy and prognosis of gastric carcinoma．