Na+／H+交换体阻滞剂对糖尿病鼠心肌细胞缺氧／复氧损伤的保护机制

Na+／H+ permutoid retarder to diabetic mouse cardiac muscle cell oxyen deficit/reaerationon injury protection mechanism discussion

关键词:  糖尿病  心肌病变  缺血/再灌注损伤  Na+／H+交换体阻滞剂

Key words:  cardiomyopathy  ischemia/reperfusion injury  Na+/H+ exchanger

摘要:

［目的］探讨Na⁺／H⁺交换体阻滞剂对糖尿病鼠心肌细胞缺氧／复氧损伤的保护机制。［方法］ 通过观察糖尿病鼠单个心肌细胞在缺氧/复氧时细胞胞浆Ca²⁺浓度的动态变化以及Na⁺/H⁺交换体阻滞剂（EIPA）在不同时相（糖尿病单纯缺氧组即DM组，缺氧/复氧全程给药组即DM-EIPA组，复氧前给药1组即DM-EIPA 1组，复氧前给药2组即DM-EIPA 2组）对Ca²⁺浓度的影响来研究Na⁺/H⁺交换体阻滞剂对糖尿病鼠心肌细胞缺氧/复氧损伤的保护机制。［结果］DM-EIPA组在180 min的缺氧期间Ca²⁺浓度的上升幅度显著低于DM组、DM-EIPA 1组和DM-EIPA 2组；在复氧期间DM-EIPA组和DM-EIPA 2组Ca²⁺浓度的上升幅度显著低于DM组,而前两组之间差异无显著性意义。［结论］EIPA可以通过减轻钙超载而对缺血再灌注的糖尿病鼠心肌细胞起保护作用，可能对糖尿病心肌病的发生发展有预防作用。

Abstract:

［Objective］To detect the protection mechanism of Na⁺/H⁺ permutoid retarder to diabetic mouse cardiac muscle cell oxygen deficit/reaeration on injury.［Methods］By observing the cytoplasmic Ca²⁺ concentration change of single cardiocyte in I/R status and the effect of Na⁺/H⁺ permutoid retarder to Ca2+ concentration in different phases (diabetes pure oxygen-lack deficit group called DM group,the oxygen deficit/reaeration on entire journey for medicine group called DM-EIPA group， the first group giringmedicine before reaeration on called DM-EIPA 1 group, and the second group giringmedicine before reaeration on called DM-EIPA 2 group) to detect the protection mechanism of Na⁺/H⁺permutoid retarder to diabetic mouse cardiac muscle cell oxygen deficit/reaeration injury.［Results］The increasing extent of Ca²⁺ concentration in DM-EIPA group was significantly smaller than that in DM group,DM-EIPA 1 group and DM-EIPA 2 group; The increasing extent of Ca²⁺ concentration in DM-EIPA group and DM-EIPA 2 group were significantly smaller than that in the DM group,but the difference was not significantly between the former two groups.［Conclusions］EIPA can protect I/R cardiocyte of the diabetic mouse by decreasing the Ca²⁺ overload, which may prevent the onset and development of diabetic cardiomypathy.