149株念株菌鉴定与药敏分析

Identity and antifungal susceptibility of 149 strains of candida

关键词:  念株菌  耐药性  药敏

Key words:  candida  drug resistance  susceptibility

摘要:

［目的］了解念珠菌感染的种类及药敏状况。［方法］ 念珠菌鉴定用API20AUX板条药敏试验用，ATB-Fungus试剂盒和纸片扩散法。［结果］ 白色念珠菌分离率最高，其次是热带念珠菌，光滑念珠菌和克柔念珠菌（以下简称白念、热带、克柔、光滑）。呼吸道感染以白念、热带为主；泌尿系感染则以光滑多见。白念、热带、克柔、光滑对氟康唑耐药率分别为4.8%、3.8%、50%和13.5%。它们对5-氟胞嘧啶、两性霉素B、制霉菌素耐药均在8%以下，对咪唑类耐药8%~15%左右。［结论］正确鉴定念珠菌对临床选择抗真菌药是极为重要的，对其耐药监测可为临床真菌的防治提供依据。

Abstract:

［Objective］To know the kinds and antifungal susceptibility of Candida .［Methods］Candida were identified with API 20AUX and antifungal susceptibility were determined by Rosco disk diffusion and brth microdilution method (ATB-Fungus )［Results］the strains of Candida albicans were the most frequent organism ,followed by C.tropicalis, C. glabrata and C.krusei. C.albicans and C.tropicalis were common in respiratory infection, C.glabrata were common in urinary infection.The resistant rates of fluconazole against C.albicans, C.tropicalis, C. glabrata and C.krusei were 4.8%, 3.8% 50% and 13.5% respectively. The resistant rates of 5-Flucyosive, amphotericin B and nystatin against Candidas were lower than 8%,and that of imidazole against Candida were between 8%~15%.［Conclusions］It is important for accurate identity Candida help selection of antifungal. The surveillance of antifungal resistance could supply bases for the prevention and treatment of Candida infection.