天然牛磺酸对肝纤维化大鼠TGF-β1/Smad信号通路的影响

Effects of natural taurine on TGF-β1/Smad singaling pathway in rats of hepatic fibrosis

关键词:  牛磺酸  肝纤维化  转化生长因子-β₁  Smad

Key words:  taurine  hepatic fibrosis  transforming growth factor-β₁   Smad

摘要:

［目的］探讨天然牛磺酸对肝纤维化大鼠TGF-β₁/Smad信号通路的影响。［方法］Wistar大鼠随机分成对照组、模型组、天然牛磺酸治疗组，采用CCL4诱导肝纤维化模型。酶联免疫吸附法（ELISA）测定血清TGF-β₁含量；免疫组织化学法检测肝组织TGF-β₁、Smad3、Smad7蛋白表达；RT-PCR检测肝组织TGF-β₁、TβRI和TβRIImRNA表达；分别用HE染色和Masson染色。肝组织观察病理组织变化。［结果］ 治疗组血清TGF-β₁含量为(1.65±0.32)μg/L,显著低于模型组(3.93±0.32)μg/L,P<0.01;治疗组肝组织TGF-β₁,Smad3蛋白表达分别为(4.5±0.6)%、(2.5±0.8)%,显著低于模型组(7.5±1.2)%、(3.9±0.8)%,P<0.05，Smad7为(6.2±1.3)%,显著高于模型组(1.3±0.2)%,P<0.01;治疗组肝组织TGF-β₁,TβRI和TβRIImRNA表达分别为0.83±0.21、0.45±0.16、0.63±0.15，显著低于模型组1.62±0.45、0.93±0.24、1.25±0.45，P<0.01；治疗组肝纤维化分期积分为2.07±0.31，显著低于模型组3.18±0.42，P<0.01。［结论］天然牛磺酸能抑制TGF-β₁及其受体和Smad3的表达，上调Smad7的表达，具有抑制肝纤维化的作用。

Abstract:

［Objective］To investigate the Effects of natural taurine on TGF-β₁/Smad singaling pathway in rats of hepatic fibrosis. ［Methods］Wistar rats were randomly divided into normal group, model group and natural taurine treatment group(each group,n=20),hepatic fibrosis model were induced by CCL4. The serum concentration of TGF-β1was assayed with ELISA.The protein expression of TGF-β₁,Smad4,Smad7, with immunohis- stochemistry method.The gene expression of TGF-β₁,TβRI and TβRII with PR-PCR method.Histopathological changes in rat’s liver tissue were detected with HE and Masson collagen staining.［Results］Treatment group of serum concentration of TGF-β₁were (1.65±0.32)μg/L ,being lower than that in model group (3.93±0.32)μg/L,P<0.01,the protein expression of TGF-β₁,Smad3 were (4.5±0.6)%, (2.5±0.8)% respectively, being lower than that in model group (7.5±1.2)%, (3.9±0.8)%,P<0.05, Smad7 were (6.2±1.3)%, being higher than that in model group (1.3±0.2)%,P<0.01, the gene expression of TGF-β₁, TβRI , TβRII were 0.83±0.21, 0.45±0.16, 0.63±0.15 respectively，being lower than that in model group 1.62±0.45, 0.93±0.24, 1.25±0.45,P<0.01. Treatment group of hepatic fibrosis score were 2.07±0.31, being lower than that in model group 3.18±0.42，P<0.01.［Conclusions］Natural taurine can inhibit the expression TGF-β₁and it’s receptor,and increase the expression Smad7 and can inhibit hepatic fibrosis.