| 摘要: |
| ［目的］观察蜂蜡膏对糖尿病新西兰兔创面愈合的影响。［方法］采用四氧嘧啶人工复制糖尿病新西兰兔模型9只，14 d后在背部各制备3个面积为2.54 cm2的全层皮肤缺损创面，将创面分为3组，即蜂蜡膏组，湿润烧伤膏组(MEBO)和空白组。通过观察残余创面面积，创面愈合时间，组织病理学量化和免疫组化结果来评价治疗效果。［结果］治疗8、12和16 d后，蜂蜡膏组创面面积较空白组明显减小（1.51±0.16）cm2、（1.80±0.11）cm2，（0.46±0.11）cm2、（1.15±0.23）cm2，（0.19±0.09）cm2、（0.59±0.19）cm2，P＜0.05，愈合时间明显缩短（18.9±1.4 ）、（22.0±2.3）d ，P＜0.01。 组织病理学检测蜂蜡膏组治疗的创面， 成纤维细胞和新生毛细血管数量与空白组比较，差异有显著性意义(60.8±6.7、50.6±11.0，P＜0.05；15.12±3.10、10.28±3.17，P＜0.01)。经免疫组织化学方法检测蜂蜡膏组增殖细胞核抗原(PCNA)的表达与空白组比较，差异有显著性意义(163.1±8.8、171.3±4.6，P＜0.05)。综合各项指标，表明蜂蜡膏组与湿润烧伤膏组药理作用强度相似。［结论］蜂蜡膏能加速糖尿病新西兰兔创面的愈合。 |
| 关键词: 蜂蜡膏 蜂蜡 创面愈合 糖尿病 新西兰兔 |
| DOI：10.11724/jdmu.2007.04.04 |
| 分类号: |
| 基金项目: |
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| Experimental study on beeswax ointment in diabetic rabbit’s model of wound healing |
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FU Hui1, WANG Xin-jian2, LU Huan-fu3, XU Qi-wei3
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1.Nanjing R&D center of Jiangsu China Tai-Tianqing Pharmaceutical Co., Ltd， Nanjing 210038, China;2.The Second Hospital Affiliated to Dalian Medical University, Dalian 116027, China;3.Department of Pharmaceutical Analysis, Dalian Medical University, Dalian 116027, China
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| Abstract: |
| ［Objective］To investigate the effect of beeswax on wound healing in diabetic rabbits.［Methods］Induced 9 diabetic rabbits through alloxan, after 14 days, three full-thickness skin wounds of 2.54 cm2 were incised in the back of each diabetic rabbit, and the wound were divided into three groups: beeswax ointment group, ointment (MEBO) group and blank control group. The wound healing effect was assessed by the measurements of wound area, the healing time of each group. The histology was observed and proliferate cellular nucleolus antigen (PCNA) was investigated quantitatively by means of immunohistochemistry.［Results］The wound area reduced obviously in beeswax ointment group after 8，12 and16 days than that of blankcontrolgroup(1.51±0.16 vs 1.80±0.11) cm2;(0.46±0.11 vs 1.15±0.23) cm2;(0.19±0.09 vs 0.59±0.19) cm2, P＜0.05. The healing time of granulation wounds was shorten obviously than that of blank control group (18.9±1.4 vs 22.0±2.3)d, P＜0.01. Histopathology showed the number of fibroblasts and new blood capillaries in beeswax ointment group were obviously improved as compared with blank control group 60.8±6.7 vs 50.6±11.0，P＜0.05；15.12±3.10 vs10.28±3.17,P＜0.01.With immunohistochemistry and image analysis, the expression of PCNA in beeswax ointment group was higher than blank control group 163.1±8.8 vs 171.3±4.6,P＜0.05. In addition, the results showed that beeswax ointment and ointment (MEBO) which was used as positive control drug had the same pharmacological potency with no statistically significant difference in the above experiments.［Conclusions］Beeswax ointment has the remarkable therapeutic effects to diabetic rabbits wound healing. |
| Key words: beeswax ointment beeswax wound healing diabetes rabbit |
