临产妇血清蛋白电泳谱分析

Electrophoresis of serum proteins in parturient women

关键词:  电泳  琼脂凝胶  血清蛋白

Key words:  electrophoresis  agar gel  serum proteins

摘要:

［目的］探讨临产妇血清蛋白电泳谱的改变。［方法］ 利用Helena全自动琼脂糖蛋白电泳仪对50例临产妇（实验组）及30名育龄非孕健康女性（正常对照组）血清蛋白电泳、总蛋白、白蛋白、高密度脂蛋白胆固醇（HDL-C）、低密度脂蛋白胆固醇（LDL-C）、载脂蛋白、血清钙同时采用散射比浊法测定了20例实验组、10例对照组血清转铁蛋白（TRF）、铜蓝蛋白（CER）、C₃、CRP、IgG、IgA、IgM。［结果］ 发现其图谱有一定的特征：α₁、α₂、β球蛋白显著增高（P＜0.01），而白蛋白、γ球蛋白降低（P＜0.01）。血清中脂类测定的结果显示高密度脂蛋白胆固醇（HDL-C）、低密度脂蛋白胆固醇、载脂蛋白、总胆固醇、甘油三脂均增高（P＜0.001）。另外，血清钙的水平以及TRF、CER、CRP、IgG、IgA的水平也增高（P＜0.01），而IgM和C₃的水平却是无显著的变化（P＞0.05）。［结论］对临产妇进行血清蛋白电泳谱分析对孕妇的临床生理研究提供一定的理论支持。

Abstract:

［Objective］To investigate the change of serum protein electrophoresis in parturient women.［Methods］The HELENA agarose gel eletrophoresis technique was used for serum protein electrophoresis, measuring of total protein albumin,HDL-c ,LDL-c ,ApoA1 and ApoB, TRF,CER,C3,CRP,IgG,IgA,IgM in both 100 parturient women(study group) and 30 healthy women(control group).［Results］Compared with the control group, the level of total protein and albumin in the study group were lower (P＜0.001), the percent of albumin was lower(P＜0.01,the percent of α1, α2 and β-globin were higher (P＜0.01), the percent of γ-globin was lower(P＜0.01);the lipids in study group were higher than that in control group conclusion The changes of serum protein electrophoresis results in the parturient women were mainly presented with the decreased in albumin and γ-globin levels,increased percent of α1, α2,and β-glibins ,increased percent of α2 and β-globins markedly related with increased a and b-lipoprotein. Agarose gel electrophoresis as a screening method for component of F is valuable.