慢性乙型肝炎患者血清e系统与HBV-DNA含量的关系及临床意义

Relation and clinical meaning of serum e system and HBV-DNA levels in patients with chronic hepatitis B

关键词:  荧光定量聚合酶链反应  乙肝病毒  核酸

Key words:  fluorescence quantitative  hepatitis B virus  DNA

摘要:

［目的］ 探讨慢性乙型肝炎患者血清HBV-DNA的表达水平与e系统的关系及其与肝细胞 损害的关系。［方法］用实时荧光定量PCR方法检测乙肝患者血清HBV-DNA，同时用ELISA法 检测血清乙肝标志物。［结果］HBeAg(+)组与抗-HBe（+）组HBV-DNA阳性率分别为100%、58 .89%，两组HBV-DNA含量比较，差异无显著性（P>0.05）;两组肝细胞损害指标ALT、AST 、TBA比较，差异有显著性（P<0.05），HBV-DNA含量与肝细胞损害各指标均无相关性。［结论］HBV-DNA的定量检测可更准确地反映体内HBV增殖复制水平，HBV-DNA的含量与肝实质损害程度无关。

Abstract:

［Objective］ To explore the relationship between the expression levels of serum HBV-DNA and the serum e system,and the degree of hepatocyte injury in patients with chronic hepatitis B.［Methods］The contents of HBV-DNA were detected by fluorescence quantitative PCR,and the HBV serum marks of immunology by ELISA.［Results］The positive rate of HBeAg(+) group and anti-HBe(+) group was respectively 100% and 58.89%,The contents of HBV-DNA copies had no significant difference between two groups (P>0.05);There had significant difference between the contents of HBV-DNA copies and the severe degree of hepatocyte injury (P<0.05);The contents of HBV-DNA copies had no correlation with the severe degree of hepatocyte injury (P>0.05).［Conclusion］The quantitative detection of serum HBV-DNA can a ccurately react the duplication level of hepatitis virus.The severe degree of hepatocyte injury has not a direct correlation with the contents of HBV-DNA copies.