应用PCR-SSCP法检测乳腺癌患者血中p53基因突变

Plasma p53 gene mutation in primary breast cancer patients

关键词:  乳腺癌  p53基因  突变  PCR-SSCP

Key words:  breast cancer  p53 gene  mutation  PCR-SSCP

摘要:

［目的］探讨乳腺癌患者外周血p53基因突变的临床意义。［方法］应用聚合酶链反应—单链构象多态性分析（PCR-SSCP）的方法检测35例乳腺癌患者和20例良性肿瘤患者外周血中p53基因外显子5～8的突变情况。［结果］乳腺癌患者p53基因突变的检出率为40.0%（14/35），显著高于对照组良性肿瘤患者的5.0%（1/20）（P＜0.05）。［结论］p53基因突变在乳腺癌外周血中有较高的检出率，是乳腺癌发生的早期信号。

Abstract:

Objective］To investigate the clinical significance about p53 gene mutation in blood of the patients with breast cancer. ［Method］Use of single strand conformation polymorphism analysis of polymerase chain reaction products(PCR-SSCP) to inspect p53 gene mutation from 35 blood samples of the patients with breast cancer and 20 blood samples of the patients with breast benign tumor.［Result］The mutation rate of the patients with breast cancer were 40.0%(14/35) ,and the mutation rate of the patients with breast benign tumor were only 5.0%(1/20).The difference was significant（P＜0.05） .［Conclusion］Blood p53 gene mutation from the patients with breast cancer have higher detection rate , which is the early period signal of breast cancer.