应用PCR-SSP方法检测HLA-B27亚型及其临床意义

Clinical implication of HLA-B27 subtypes by polymerase chain reaction with sequence specific primers

关键词:  PCR-SSP  HLA-B27亚型  强直性脊柱炎

Key words:  PCR-SSP  HLA-B27 subtype  ankylosing spondylitis.

摘要:

［目的］通过PCR-SSP方法对HLA-B27亚型进行检测，探讨其与强直性脊柱炎（AS）的相关性，并对大连地区B-27分型情况进行研究。［方法］对40例确定为HLA-B27阳性的样本(临床诊断28例AS,12例非AS)采用序列特异性引物-聚合酶链反应（PCR-SSP）检测方法进行HLA-B27亚型分型。［结果］40例样本用PCR-SSP方法检测均获成功。共检出4种亚型：B2704、B2705、B2702和B2710。 B2704 23例占57.5%(23/40例)；B2705 14例占35%(14/40例)；B2702 2例占5%(2/40例)；B2710 1例占2.5%(1/40例)。28例AS标本与12例非AS标本中B2704、B2705所占比例统计学分析无显著差异(B2704χ2=0.5894, P>0.05；B2705χ2 =0.02, P>0.05)。 ［结论］PCR-SSP方法对HLA-B27基因分型,分辨度高、特异性强、快捷、准确,适合于临床应用。HLA-B27亚型的检测可作为强直性脊柱炎诊断（AS）的重要的参考指标。大连地区HLA-B27亚型以B2704和B2705为主并出现1例B2710型。

Abstract:

［Objective］ To detect the HLA-B27 subtypes by Polymerase Chain Reaction with sequence specific primers. To study the clinical meaning of HLA-B27 subtypes associated with ankylosing spondylitis (AS) and in vestigate HLA-B27 subtypes of the Dalian area. ［Methods］ Subtyping for HLA-B27 of Polymerase Chain Reaction with sequence specific primers(PCR-SSP) method was applied. A total of 40 HLA-B27 positive samples were entered into the study, containing 28 samples diagnosed ankylosing spondylitis and 12 samples diagnosed not ankylosing spondylitis. ［Results］ Subtyping for B27 was successfully tested in 40 samples by PCR-SSP.Checkout four HLA-B27 Subtypes:B2704, B2705,B2702 and B2710. B2704 accounting for 57.5% in all 40 samples,B2705 accounting for 35%. There was no significant difference between the rate of B2704,B2705positive sample in ankylosing spondylitis and not ankylosing spondylitis (B2704χ2=0.5894,P>0.05; B2705χ2=0.02,P>0.05).［Conclusion］ Subtyping for HLA-B27 by PCR-SSP has proved to be a high-sensitivity, high-specificity, rapid and accurate technique , suitable for clinical application. It is necessary to diagnose ankylosing spondylitis in clinic.The dominating subtype for HLA-B27 of Dalian area is B704 and B2705,only finding B2710 one example.