hMSH2蛋白和P53蛋白表达在胃癌发病中的意义

Significance of gene product expressions of hMSH2 and P53 in gastric carcinogenesis

关键词:  错配修复基因  hMSH2  抑癌基因  p53  胃癌

Key words:  mismatch repair gene  hMSH2  tumor suppressor gene  p53  gastric carcinoma

摘要:

［目的］探讨DNA错配修复基因hMSH2蛋白、抑癌基因P53蛋白表达在胃癌发病中的意义。［方法］ 采用免疫组织化学技术（S-P法）检测63例胃癌组织及32例胃炎组织hMSH2、P53的表达。［结果］ 胃癌组hMSH2蛋白阳性表达率（79.37%）明显高于胃炎组（53.13%）（P<0.05）；胃癌组P53蛋白阳性表达率（88.89%）明显高于胃炎组（21.88%）（P <0.05）。hMSH2蛋白在高中分化腺癌、低分化腺癌及黏液癌的阳性表达率分别为73.68%、82.76%和80.00%，P53蛋白阳性表达率分别为100.00%、89.66%和73.33%，两者阳性表达率与胃癌分化程度均无显著性差异（P>0.05）。hMSH2蛋白和P53蛋白表达呈正相关。［结论 ］ DNA错配修复基因hMSH2蛋白及抑癌基因P53蛋白的异常表达可能参与胃癌发生机制。

Abstract:

［Objective］ To study the relationship of expressions of gene product hMSH2, P53 to gastric carcinogenesis. ［Method］ Immunohistochemical technique was used to detect hMSH2 and P53 protein expressions in 63 cases of gastric carcinomas and 32 cases of gastritis. ［Results］ The positive rate of hMSH2 in gastricc arcinoma being 79.37%(50/63) was significantly higher than that in gastritis being 53.13%(17/32) (P<0.05); the positive rate of P53 in gastric carcinoma being 88.89% (56/63) was significantly higher than that in gastritis being 21.88%(7/32) (P<0.05). The positive rates of hMSH2 in well medial differentiation,poordifferentiation and mucoid carcinoma were 73.68% , 82.76% and 80.00% respectively（P>0.05）, and the positive rates of P53 were 100.00%, 89.66% and 73.33% respectively（ P>0.05）. The expression of hMSH2 was positively correlated to the expression of P53 in gastric mucosa including carcinoma and gastritis (r=0.223, P<0 .05). ［Conclusion］ The abnormal expressions of mismatch repair gene hMSH2 and tumor suppressor gene P53 may play some roles in the gastric carcinogenesis.