胃癌组织hMSH2基因突变的检出

Detection of hMSH2 gene mutation in gastric carcinoma

关键词:  错配修复基因  hMSH2  胃癌

Key words:  mismatch repair gene  hMSH2  gastric carcinoma

摘要:

［目的］研究hMSH2基因表达及其突变在胃癌发生中的作用。 ［方法］应用反转录—聚合酶链反应（RT-PCR）技术检测24例胃癌组织及15例癌旁组织的 hMSH2 mRNA表达情况。［结果］胃癌组的hMSH2 mRNA检出率为50%（12/24），高于癌旁胃黏膜组的20%（3/15）；胃癌组织突变检出率为20.8%（5/24），高于癌 旁胃黏膜的0%（0/15）；5例突变中，杂合性突变2例，纯合性突变3例。 在配对的15例中，仅胃癌组检出突变3例，均为纯合性突变。［结论］胃癌的发生伴随着hMSH2 基因的表达上调，这种表达上调可能与该基因突变有关。

Abstract:

［Objective］ To study the roles of hMSH2 gene expression and mutation in gastric carcinogenesis. ［Method］ Reverse transcription polymerase chain reaction (RT-PCR) was used to examine the expressions and mutations of hMSH2 gene in 24 cases of gastric carcinoma tissues in comparison with those in 15 cases of their peri-carcinoma tissues. ［Results］ The detective rate of hMSH2 mRNA in gastric carcinoma tissues being 50% (12/24) was higher than that in peri-carcinoma tissues being 20% (3/15). The detective rate of hMSH2 gene mutation in gastric cancer tissues being 20.8% (5/24) was higher than that in peri-carcinoma tissues being 0% (0/15). In the 5 mutational cases, there were 2 heterozygous mutations and 3 homozygous mutations. And in the 15 paired cases, 3 homozygous mutations were only found in gastric carcinoma tissues. ［Conclusion］ The up-regulated hMSH2 mRNA expressions, which may related to the mutation of the hMSH2 gene, associated with gastric carcinogenesis.