| 摘要: |
| ［目的］探讨中药四君子汤对膀胱癌荷瘤小鼠化疗中免疫功能的保护作用。［方法］用小鼠膀胱癌细胞接种于T739小鼠皮下，接种24 h 后分别给药，动物随机分为8组，对照组，丝裂霉素C(MMC)组，大剂量四君子汤组，中剂量四君子汤组，小剂量四君子汤组，大剂量四君子汤+MMC，中剂量四君子汤+MMC，小剂量四君子汤+MMC。用药7 d 后检测各组小鼠外周血中的白细胞数、淋巴细胞转化率，用药14 d后测定小鼠的脾重、NK细胞活性。［结果］四君子汤的3个剂量组均能使化疗时荷瘤小鼠的血白细胞数、脾重、NK细胞活性维持在正常水平，与MMC组比较差异有显著性(P<0.05)；大剂量四君子汤组的淋巴细胞转化率及脾重最高，达到(41.2±4.2)% 及(206.8±4.1) mg，与其他各组比较差异有显著性(P<0.05)；大剂量四君子汤+MMC组的各项检测指标均优于中或小剂量四君子汤+MMC及MMC组, 组间比较差异均有显著性(P<0.05)。［结论］ 四君子汤可增强荷瘤小鼠化疗时的机体免疫功能。 |
| 关键词: 四君子汤 膀胱癌 化疗 NK细胞 淋巴细胞转化率 |
| DOI：10.11724/jdmu.2006.03.02 |
| 分类号:R737.14 |
| 基金项目: |
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| Immunoprotective effects of sijunzi decoction on bladder carcinoma treated by chemotherapy in mice |
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LI Chuan-gang1, LI Mo-lin2, SHU Xiao-hong3, JIA Yu-jie2, LIU Yong-ji1
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1.Department of Surgery, the Second Affiliated Hospital, Dalian Medical University, Dalian 116027, China;2.Department of Pathophysiology,Dalian Medical University, Dalian 116027, China;3.Department of Pharmaceutical Chemistry, Dalian Medical University, Dalian 116027, China
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| Abstract: |
| ［Objective］Investigating the immunoprotective effects of Sijunzi decoction (SJZD) on bladder carcinoma treated by chemotherapy in mice.［Methods］T739 mice were inoculated subcutaneously with mouse bladder carcinoma cells, and then randomly divided into 8 groups: control group, mitomycin C (MMC) group, high-dosage SJZD group, medium-dosage SJZD group, low-dosage SJZD group, MMC with high-dosage SJZD group, MMC with medium-dosage SJZD group, and MMC with low-dosage SJZD group respectively. The medication was begun in 24 h after tumor cell inoculation. The number of white blood cells in peripheralblood and the change of transformation efficiency of lymphocyte (LT) were determined 7 days after treatment. The NK cell activities and spleen weight of the mice were determined 14 days aft
er treatment.［Results］All data from three SJZD groups of mice were in the normal range. Compared with the data from MMC group of mice, the difference is significant (P<0.05). The LT and spleen weight were (41.2±4.2)% and (206.8±4.1) mg respectively in the group of mice with high dose of SJZD treatment, it was obviously different to that in the other groups of mice (P<0.05). All data from the group of mice treated with MMC and high-dosage SJZD group were better than those from the
groups of mice treated with MMC and medium or low dosage SJZD and MMC group (P<0.05). ［Conclusion］ SJZD could elevate the immune function of tumor-bearing mice treated with chemotherapy. |
| Key words: sijunzi decoction bladder carcinoma chemotherapy NK cells transformation efficiency of lymphocyte |
