全身炎性反应综合征时中性粒细胞凋亡的异常

Dysregulation of polymorphonuclear neutrophil apoptosis in systemic inflammatory response syndrome

关键词:  全身炎性反应综合征  中性粒细胞  细胞凋亡

Key words:  systemic inflammatory response syndrome  polymorphonuclear neutrophil  apoptosis

摘要:

［目的］研究中性粒细胞即多形核白细胞（polymorphonuclear neutrophil，PMN）凋亡的异常在全身炎性反应综合征(systemic inflammatory response syndrome, SIRS)中的意义。［方法］ SIRS患者8例（均为急性胰腺炎患者）为SIRS组，正常人6例为对照组。采用流式细胞仪和TUNEL方法定量检测外周血PMN的凋亡情况，采用ELISA法检测血清IL-6、IL-8的水平。［结果］流式细胞仪检测：SIRS组外周血PMN的细胞凋亡率为（42±8）%，低于对照组的（63±9）%，P<0.05； TUNEL法检测：SIRS组外周血PMN的凋亡指数（apoptosis index，AI）为25±7，低于对照组的37±10，P<0.005。SIRS组外周血血清IL-6水平为（8.2±2.2）pg/mL，高于对照组的（2.1±1.3）pg/mL，P<0.001；SIRS组外周血血清IL-8的水平为（38±12）pg/mL，高于对照组的（12±4）pg/mL，P<0.001。［结论］SIRS患者存在PMN的凋亡延迟，其在SIRS的发生、发展中有着重要的意义。

Abstract:

［Objective］To study the dysregulation of the polymorphonuclear neutrophil (PMN) apoptosis in the systemic inflammatory response syndrome (SIRS), and the clinical significance. ［Methods］ Eight acute pancreatitis patients with SIRS, 6 healthy control subjects were observed about the following: apoptosis of PMN in peripheral blood samples analysed by flow cytometry and TUNEL; the values of serum IL-6、IL-8 detected by ELISA. ［Results］ Spontaneous apoptosis was significantly delayed in PMN from patients with SIRS compared with controls (42±8)% vs (63±9)% by flow cytometry, P<0.05 and 25±7 vs 37±10 by TUNEL, P<0.005. The levels of IL-6 in the SIRS patients was higher than that of controls （8.2±2.2）pg/mL vs （2.1±1.3）pg/mL, P<0.001, the levels of IL-8 in the SIRS patients was higher than that of controls （38±12）pg/mLvs （12±4）pg/mL, P<0.001. ［Conclusions］ Circulating PMN from acute pancreatitis patients with SIRS show delayed apoptosis, and prolonged PMN survivalmay contribute to the systemic inflammatory injury characteristic of SIRS.