转化生长因子β1、I型胶原在Borrmann Ⅳ型胃癌形成中的作用研究

Function of TGF-β1 and collagen type Ⅰexpression in formation of Borrmann type Ⅳ gastric carcinoma

关键词:  转化生长因子β1  Ⅰ型胶原  胃肿瘤  免疫组织化学 Borrmann分型

Key words:  transforming growth factorβ1  collagen type Ⅰ  stomach neoplasm  immunohistochemical  borrmann classification

摘要:

［目的］分析转化生长因子β1（TGF-β1）表达与胃癌肉眼形态的关系及其在Ⅰ型胶原（CollagenⅠ）合成中的作用，初步探讨TGF-β1与Ⅳ型胃癌形成的关联。 ［方法］ 通过免疫组织化学染色SP方法，分析了109例胃癌患者原发灶中TGF-β1及CollagenⅠ的表达。［结果］ TGF-β1表达分级在Borrmann分型各型间有显著性差异(P<0.005), Borrmann Ⅳ型胃癌TGF-β1呈++～+++级表达的占96%,而在Ⅰ、Ⅱ、Ⅲ型中分别为38.46%、76.31%、81.82%；TGF-β1表达与CollagenⅠ表达有相关关系(P<0.005)；CollagenⅠ表达在Borrmann分型各型间有显著性差异(P<0.001)，Borrmann分型为Ⅰ、Ⅱ、Ⅲ、Ⅳ型的CollagenⅠ表达面积比分别为8.22±0.81、8.69±0.90、11.20±1.45、14.27±1.71。Ⅳ型与Ⅰ、Ⅱ、Ⅲ型之间均有显著性差异（P<0.001）。［结论］ TGF-β1表达与CollagenⅠ表达有明显相关性，在以胃壁广泛纤维化为特点的Borrmann Ⅳ型胃癌形成过程中起促进作用。

Abstract:

［Objective］ To investigate the expression of TGF-β1 in different type of Borrmann classification gastric carcinoma and it's effect on the synthesis of collagen type Ⅰ,hope to find the correlation between TGF-β1and the formation of Borrmann type Ⅳ gastric carcinoma. ［Methods］ One hundred and nine formalin-fixed、paraffin-embeded sections(4 μm) from resected gastric carcinomas were subjected to immunostaining using Streptavidin—Peroxidase technique to analyse the expression of TGF-β1 and collagen type Ⅰ. ［Results］ TGF-β1 expression was positively correlated with Borrmann classification(P<0.005).The expression degree of TGF-β1 with II～III in Borrmann type Ⅳ gastric carcinoma was 96%,but in Borrmann type I，II，III were 38.46%，76.31%，81.82% respectively. We found significant correlation between collagen type Ⅰ expression and TGF-β1expression(P<0.005), We also found collagen type Ⅰ expression closely correlated with Borrmann classification(P<0.001). The expression proportion of collagen type Ⅰin borrmann type I，II，III，IV were 8.22±0.81， 8.69±0.90， 11.20 ±1.45， 14.27±1.71 respectively. Significant correlation was found between Borrmann type Ⅳand I，II，III(P<0.001). ［Conclusions］ The expression of TGF -β1 is clossly correlated with the expression of collagen type Ⅰ,so it might play a important role in the formation of Borrmann type Ⅳ gastric carcinoma which is characterised by extensive fibrosis in gastric wall.