早期胃癌及癌旁组织P53及PCNA蛋白表达的意义

Significance of expression of P53 and PCNA proteins in primary gastric cancer and para-cancerous tissue

关键词:  早期胃癌  PCNA（增殖细胞核抗原）  抑癌基因蛋白P53

Key words:  primary gastric cancer  poliferating cell nuclear antigen(PCNA)  P53 protein

摘要:

［目的］探讨抑癌基因蛋白P53及PCNA蛋白在早期胃癌发生中所起的作用。［方法］ 应用免疫组化技术检测28例早期胃癌及其癌旁组织的PCNA及抑癌基因蛋白P53的表达情况，采用t检验和χ2检验统计分析。［结果］（1）正常胃黏膜、肠上皮化生黏膜、异型增生病灶及早期胃癌的PCNA阳性细胞率分别为11.7%、20.3%、36.4%和49.2%，细胞增殖活性逐渐增高，各组间有显著差异(P<0.05)；在异型增生黏膜中，随异型程度的增高，增殖活性也逐渐升高，轻度、中度、重度病变的PCNA阳性细胞率分别为29.4%、35.5%和43.2%，各组与癌灶的PCNA阳性细胞率（49.2%）相比有显著差异(P<0.05)。（2）抑癌基因蛋白P53在轻度、中度、重度异型增生及早期胃癌中可见表达，阳性率分别为11.1%、14.3%、30.0%和42.4%，各组间相比无显著差异(P>0.05)；而正常胃黏膜、肠上皮化生黏膜P53未见表达。（3）抑癌基因蛋白P53阳性表达的病例PCNA阳性细胞率明显高于P53阴性表达的病例(P<0.05)。［结论］ PCNA及抑癌基因蛋白P53在胃癌的发生中起重要的作用。

Abstract:

［Objective］ To study tumour suppressor gene P53 protein and PCNA protein expression in primary gastric cancer and define whether they were associated with the stomach carcinogenesis.［Methods］ This article examines P53 and PCNA protein expression in 28 cases of primary gastric cancers and para-cancerous tissue by immunohistochemistry. ［Results］ (1)The percentages of PCNA-positive cells from normal (11.7%)，intestinal metaplasia (20.3%) and dysphasia (36.4%) to early gastric cancer (49.2%) increases gradually， and the differences among them is very obvious (P<0.05)； As to dysphasia，the proliferative activity from mild (29.4%) and moderate (35.5%) to severe (43.2%) also increase gradually. There is apparent difference between each of them and primary cancer (P<0.05).(2) Positive staining of P53 protein only appears in the dysplasia and cancer. As to dysplasia，when the degree increases， positive staining of P53 protein also increases with it(mild，11.1%；moderate，14.3%；severe，30%)；There is no apparent difference between severe dysplasia(30%) and cancer(42.4%)(P>0.05).(3)The proliferative activity of P53-positive cases is much higher than that of P53-negative cases. ［Conclusion］ Tumour suppressor gene P53 protein and PCNA take the important roles.