大鼠羊水栓塞时的内皮素 -1 的含量及其作用的研究

Study on contect and action of endothelin-1 in amniotic fluid embolism of rat models

关键词:  羊水栓塞  内皮素  颈动脉血压

Key words:  amniotic fluid embolism  endothelin-1  carotid blood pressure

摘要:

［目的］利用放射免疫（RIA）法测定羊水栓塞（AFE）大鼠模型血及肺组织中的内皮素I（ET-1）的含量。进一步探讨羊水栓塞的发病机机制，为寻找新的治疗方法提供理论基础。［方法］取SD雌性未孕大鼠60只，随机分成6组，每组各10只，羊水分别取自足月妊娠胎粪污染组、原羊水组、上清液组，20妊娠周组，生理盐水对照组①及未经注入任何液体的对照组②。［结果］胎粪液组、原羊水组及上清羊水组ET-1的水平与对照组存在着显著的差异，胎粪组血清ET-1水平为147.79PG/mL，上清液组为83.98PG/mL。20妊娠周羊水组ET-1水平与对照组间无显著差异。［结论］内皮素与羊水栓塞的发生存在着密切关系。内皮素随着羊水栓塞病情的加重而增高，呈正相关。

Abstract:

［Objective］To determine the acute-phase concentrations of ET-1. To discuss the physiology and pathophysiology of AFE. And act basis for finding methods to treat AFE.［Methods］ Sixty SD femal nonpregnant rats were randomly as signed to one of six groups: raw amnilotic fluid; supermatant amnilotic fluid; meconium-stained fluid; 20-week amniotic fluid;control NS① and normal control②. Each group was 10rats. The concentrations of ET-1 levels in plasma and pulmon artery injection the substances were measured by radioimmunoassay. The chang of carotid blood pressure were measured by carotid intubation. ［Results］The concentrations of ET-1 in plasma and pulmon. (1) The levels of ET-1 were elevated remarkable in groups of meconium-stained fluid, raw amniotic fluid and supernatant fluid than those of control NS① and normal control②(P<0.05), and in pulmon the levdls of ET-1 in groups of meconium-stained fluid were significantly higher than those of raw amniotic fluid and supernatant fluid (P<0.05). the meconium group is 147.79 PG/ml. the control group is 83.98 PG/ml. The ET-1 levels in 20-week amniotic fluid were not higher obviously than those in control groups. And the ET-1 levels were almost equal between the two control groups. (3) There was a significant positive correlation at the ET-1 levels in plasma and pulmon(P<0.01). ［Conclusion］ The concentrations of ET-1 in plasma maybe become an important factor for the early diagnosis of AFE.