| 摘要: |
| ［目的］探讨一氧化氮（nitric oxide，NO）、过氧亚硝基阴离子（peroxynitrite ，ONOO-）在大鼠离体肾缺血再灌注损伤时对肾功能的影响。［方法］应用离体灌流肾(is olated perfused kidney，IPK)技术观察肾I-R对菊粉清除率（Cin）、尿钠排泄指数（FENa ）、 肾血管阻力（RVR）的影响；阻断内源性NO生成对肾I-R所致的上述指标变化的影响；外源性 直接给予NO供体硝普钠（SNP）、ONOO-对大鼠IPK肾功能的影响。［结果］①缺血再灌注 导 致大鼠IPK肾血管阻力（RVR）明显增大（P<0.05），IPK的Cin降低（P<0.05），肾 I-R后IPK的FENa有较大幅度的增加（P<0.05）。②SNP能够使健康大鼠IPK的RVR降低、C in增加和FENa增大（P<0.05）。而ONOO-使RVR增高、Cin降低和FENa增加（P<0.0 5）。③iNOS抑制剂氨基胍（aminoguanidine，AG）使大鼠IPK的功能I-R后5 h RVR降低、Ci n增大和尿钠减少（P<0.05）；而应用NOS抑制剂N-硝基-L-精氨酸（L-NNA），加重了肾 I-R大鼠IPK肾机能的损伤，I-R1h和I-R 5 h均出现了RVR增大、Cin降低和尿钠排泄增多（P<0.05）。［结论］肾I-R所致NO大量生成后产生的ONOO-损伤了大鼠的肾功能。 |
| 关键词: 肾 缺血再灌注损伤 离体灌流肾 一氧化氮 过氧亚硝基阴离子 氨基胍 肾功能 |
| DOI：10.11724/jdmu.2005.04.05 |
| 分类号:R692.5 |
| 基金项目: |
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| Effects of nitric oxide and peroxynitrite on renal function in isolated perfused kidney after renal I-R |
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WANG Xin-liang1, DUAN Hui-jun2, CHEN Xiao-ling3, HUANG Shan-sheng3
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1.Department of Pediatric of Second Hospital, Hebei Medical University, Shijia zhuang 050000, China;2.Department of Pathology, Hebei Medical University, Shij iazhuang 050017, China;3.Department of Pathophysiology, Hebei Medical University , Shijiazhuang 050017, China
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| Abstract: |
| ［Objective］To investigate the effects of nitric oxi de (NO) and peroxy nitrite on renal function in isolated perfused kidney(IPK) after renal I-R. ［Me thods］Isolated perfused kidney (IPK) was used to examine the effects of renal I -R on renal vascular resistance (RVR), glomerular filtration rate (GFR), fractio nal excretion of sodium (FENa); and the effects of NOS inhibition on the changes of those parameters caused by renal I-R; and the effects of SNP and ONOO- on the changes of those parameters caused by renal I-R.［Results］①RVR was increas ed, GFR was decreased, FENa was enhanced after renal I-R（P<0.05）in IPK.②S NP results in reduction of RVR, increasing of Cin and increment of FENa(P<0. 05). ONOO- (40 μmol/L) caused RVR heightened, Cin decreased and FENa increase d(P<0.05). ③RVR reduced、Cin increased and FENa enhanced(P<0.05) in I-R 5h i n IPK given AG; but L-NNA aggravated the renal injuries caused by renal I-R, exh ibited augmented R VR, reduced Cin and enhanced FENa both in I-R1h and I-R5h(P<0.05).［Conclusi on］It is proved in IPK model that a massive quantity of NO and the generation o f ONOO- play important role in renal damage of rats after renal I-R. |
| Key words: renal ischemia reperfusion isolated perfused kidn ey nitric oxide peroxynitrite aminoguanidine renal function |
