环氧氯丙烷法活化琼脂糖凝胶及其动力学分析

Sepharose activation of epichlorohydrin and its kinetic model establishment

关键词:  环氧氯丙烷  活化  琼脂糖凝胶  动力学模型

Key words:  epichlorohydrin  activation  sepharose  kinetic model

摘要:

［目的］探索环氧氯丙烷法活化琼脂糖凝胶的最佳反应条件，建立活化反应动力学模型。［方法］研究了活化反应中氢氧化钠、环氧氯丙烷和硼氢化钠的浓度以及反应温度、时间和溶剂对活化反应的影响。通过测定活化反应中环氧基的浓度，计算反应的活化度，以确定环氧氯丙烷活化琼脂糖凝胶的最佳反应条件。对影响活化反应的主要因素进行分析，建立活化反应动力学模型。［结果］ 环氧氯丙烷法活化琼脂糖凝胶的最佳反应条件为：5 g琼脂糖、7.5 mL 0.8 mol/L 氢氧化钠、2 mL环氧氯丙烷以 及10 mg硼氢化钠于25℃反应8 h。根据活化反应动力学模型推导出了反应过程的宏观动力学公式。［结论］ 对影响活化反应的主要因素进行了分析，确定了环氧氯丙烷活化琼脂糖凝胶的反应条件。将琼脂糖凝胶活化实验结果与动力学公式计算结果相比较，发现二者基本一致。

Abstract:

［Objective］ To explore the optimal condition of sepharose activation by means of epichlorohydrin and establish the kinetic model of activation process. ［Methods］ The concentrations of sodium hydroxide, epichlorohydr in and sodium borohydride, and the influences of temperature, time and solvent to activation are reviewed. The optimal condition was determined by mensurating the concentration of oxirane group and calculating activation degree. By analysis of factors, influencing reaction a kinetic model to describe the activation process was established.［Result］ The optimal condition of sepharose activation of epichlorohydrin was as followed: the mixture of 5 g sepharose, 7.5 mL 0.8 mol/L sodium hydroxide, 2 mL epichlorohydrin, and 10 mg sodium borohydride was reacted at 25℃ for 8 h. A macroscopical kinetic formula was deduced from the kinet ic model of activation process. ［Conclusion］The optimal condition was determin ed for the activation of sepharose of epichlorohydrin. The calculating result by the kinetic model was found to have good agreement with experimental result.