| 摘要: |
| ［目的］ 研究重组人干扰素α-2b（recombined human Interferon α-2b, rhIFNα-2b）含片对小鼠免疫功能的影响。［方法］ 应用比色法、计数法及ELISA双抗体夹心法，分别测定在高（50 000 IU/只）、中（5 000 IU/只）、 低（500 IU/只）3种剂量rhIFNα-2b含片作用下，不同时间（10、20、30 d）小鼠脾脏NK细 胞毒指数、腹腔巨噬细胞吞噬百分率及吞噬指数和唾液IgA 含量的变化。［结果］ 20 d各 剂量组NK细胞毒指数分别为(75.38±11.25)、(80.75±9.86)、(77.23±13.10)%，高于 空白基质片组（46.95±10.42）% (P＜0.001)； 20 d各剂量组吞噬指数分别为(0.619 ±0.054)、(0.664±0.046)、(0.599±0.038)%，高于空白基质片组（0.396±0.021）%（P＜0.001）；20 d 各剂量组唾液IgA含量分别为(70.88±10.38)、(66.28±8.98)、(54.4 1±6.35)μg/mL ，高于空白基质片组（35.67±3.51)μg/mL （P<0.001）。［结论］ rhIFNα-2b含片具有提高小鼠免疫功能的作用。 |
| 关键词: 重组人干扰素α2b NK细胞 巨噬细胞 IgA |
| DOI：10.11724/jdmu.2005.04.03 |
| 分类号:R392 |
| 基金项目: |
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| Effect of contain of recombinant human interferon α2b （rhIFN α-2b）on immune function of mouse |
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MA Tian-shu1, GE Ying-chun1, REN Hui-jun1, LIU Ping1, TIAN Jia n-ming2, ZHANG Yin-ping3
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1.Department of Pharmacology, Institute of Traditional Chinese Medi cine of Dalian, Dalian 116013, China;2.Department of Phytochemistry, Academy of Traditional Chinese Medicine and Matera Medical of Jilin Province, Changchun 13 0021, China;3.Department of Medicament, the Second Affiliated Hospital of Dali a n Medical University, Dalian 116027, China
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| Abstract: |
| ［Objective］ To study the effect of the contain of recombinant human interferon α-2b （rhIFNα-2b）on the immune function of mouse. ［Methods］ Cytotoxic index of the NK cells, phagocytic percentage and index of the abdomen macropha ges and IgA level in saliva in mouse treated with high-,moderate- or low-dosage of rhIFNα-2b(50 000,5 000,500 IU per mouse) were determined at different times (10 d, 20 d, 30 d) by colorimetry, count and sandwich ELISA, respectively. ［Results］ Compared with the blank matrix group,the cytotoxic index of 20 days is ( 46.95±10.42)%, the cytotoxic index of the NK cells of the three dosage groups of 20 days are (75.38±11.25),(80.75±9.86),(77.23±13.10)% respectively(P<0.001). Compared with the blank matrix group (the phagocytic index of macrophages of 20 days is 0.396±0.021), the phagocytic index of macrophages of the three dosage g roups of 20 days are (0.619±0.054), (0.664±0.046), (0.599±0.038) respectively(P<0.001). Compared with the blank matrix group, the IgA level in saliva of 20 days is (35.67±3.51) μg/mL the IgA level in saliva of the three dosage groups of 20 days are (70.88±10.38),(66.28±8.98), (54.41±6.35) μg/mL res pectivel y(P<0.001). ［Conclusion］ The contain of rhIFNα-2b has a significant role on enhancing the immune function of mouse. |
| Key words: rhIFNα-2b NK cells macrophages IgA |
