应用不同方法检测P糖蛋白在肺癌组织中的表达

To analyses expression of Pgp in lung neoplasms with different method

关键词:  肺癌  多药耐药性  免疫组织化学法  RT-PCR  流式细胞仪检测术

Key words:  lung neoplasms  MDR  IH  FC  RT-PCR

摘要:

［目的］探讨应用不同方法检测P糖蛋白（P-glycoprotein， Pgp）在不同人群下不同组织类型和分化程度肺癌组织中的表达情况。［方法］收集肺癌组织标本416例，显微镜下观察肿瘤的组织类型和分化情况，将肺癌组织按照病理切片情况分组，然后分别用免疫组织化学法、RT-PCR和流式细胞仪检测术检测P糖蛋白在肺癌组织中的表达情况。［结果］免疫组织化学法、RT-PCR和流式细胞仪检测术检测阳性结果分别是37％、58％和52％。Pgp阳性结果在高年龄组和复诊组较其他组别高(P＜0.05)。在不同组织类型和分化程度肺癌组织中无差别。［结论］在同免疫组织化学法比较上看，RT-PCR和流式细胞仪检测术方法简单、快速、灵敏和特异，可用于临床检测 肺癌组织中P糖蛋白。P糖蛋白在肺癌组织中的表达程度与年龄和初/复诊等情况有关而与组织类型和分化程度无关。

Abstract:

［Objective］To try to analyze the expression of Pgp in lung neoplasms by three methods.［Method］To obtained 416 surgical biopsy specimens, the expression of Pgp in lung neoplasms was determined by IH,FC and RT-PCR.［Results］The positive result were 37%, 58% and 52% by IH,CF and RT-PCR methods. The positive rate of Pgp expression was significantly higher in the elden group and re-diagnosis group than the other group (P<0.05). There is not significant difference in pulmonany pathological score. ［Conclusion］The method of FC and RT-PCR were simple;rapid;sensitive and specific;and could be used for detecting Pgp in clinic.The expression of Pgp in lung neoplasms were significantly associated with ages and second treatment ;and were not significantly associated with lung pathological sore.