细胞因子激活的脐血单核细胞对K562细胞株杀伤作用的观察

Cytotoxicity of cord blood monocyte - macrophage activated by cytokines against K562 cell line

关键词:  单核细胞  K562细胞株  IL-2  IFN-α

Key words:  monocyte-macrophage  K562 cell line  L-2  INF-α

摘要:

观察及评价白细胞介素2（IL-2）及干扰素－α（IFN-α）激活的脐血单核细胞对白血病细胞株K562 的杀伤作用。［方法］ 将细胞因子激活后的各组单核细胞(Mφ)与K562细胞株按一定效靶比作用48 h，采用MTT法检测各组的细胞毒效应。［结果］ 单用IL-2和单用IFN-α刺激的脐血单核细胞与K562细胞株作用(效靶比20∶1), 其杀伤率分别为（8.31±1.32）%、（8.75±0.85）%。IL-2联合IFN-α激活脐血单核细胞对细胞株的杀伤作用明显增强,效靶比20∶1与50∶1时，杀伤率为（41.52±2.33）%（52.66±3.50）%，与单用IL-2及单用IFN-α组比较,具有显著性差异，P＜0.01，并提示杀伤率随效靶比的增加而增加。 ［结论］ IFN-α和IL-2两者联合激活后的脐血Mφ对白血病细胞株的杀伤作用明显增强,杀伤率呈效靶比依赖关系。采用激活的脐血Mφ可用于干细胞移植时清除自体骨髓和外周血内的微小残留病变

Abstract:

To evaluate the cytotoxicity of cord blood monocyte- macrophage(Mφ) activated by interleukin-2(IL-2) and interferon-α(INF-α) and IL-2 in conbination with INF-α against K562 leukemic cell line.［Methods］ The cytotoxicity of Mφ was measured with MTT assay.［Results］ Mφ activated by 1 000 u/mL IL-2 alone and 1　000 u/mL INF-α alone produced significant cytotoxic activities against K562 cell, which were (8.31±1.32)% and (8.75±0.85)%, respectively(ratio of effector/target was 20∶1). Moreover, the same dose of IL-2 in conbination with INF-a could enhance the significant cytotoxic activities of Mφ, which was (41.52±2.33)% against K562. When the ratio of effector/target was 50∶1,the cytotoxicity was （52.66±3.50）%. With the ratio of effector/target increasing ,the cytotoxic activities of Mφ were enhanced, P＜0.01.［Conclusions］ The antitumor activity of Mφ could be further increased when IL-2 was in conbination with INF-a. And with the ratio of effector/target increasing ,the cytotoxicity of Mφ are enhanced. These results indicate that activaty cord blood monocyte-macrophage could be applied to get rid of micro-remain disease of leukemia and other malignant hematologic diseases