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| 胰岛素对糖尿病大鼠骨代谢的影响 |
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张春玉1,2, 王波2, 吕申1,2, 李淑芬1, 闻立荣1
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1.大连医科大学,第二临床学院,辽宁,大连,116027;2.大连医科大学病理学教研室,辽宁,大连,116027
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| 摘要: |
| 观察糖尿病大鼠应用胰岛素治疗后骨组织形态学改变以及血中Ⅰ型胶原氨基端肽（PINP）水平变化。［方法］3月龄雄性SD大鼠30只，随机分为糖尿病组(D)，胰岛素治疗组(I)，空白对照组( C )，每组10只。D组和I组大鼠经鼠尾静脉注射链尿佐菌素造成糖尿病大鼠模型，C组大鼠注射生理盐水。 I组大鼠分别接受中效胰岛素治疗(6～8 u/d)， 每天两次。9周及14周分别处死大鼠，取骨与血行骨组织形态计量学分析及应用放免方法检测PINP水平。［结果］9周末，D组和I组光镜下见骨质疏松表现，但3组PINP水平无差异。14周末D组仍见骨质疏松征象，而I组微观结构较9周末明显恢复；D组与C组PINP水平无差异，I组PINP水平显著增高。［结论］胰岛素可以促进糖尿病大鼠骨质的形成，抑制糖尿病大鼠骨质疏松的发生发展。 |
| 关键词: 糖尿病大鼠 骨质疏松 骨形态计量学 胰岛素 Ⅰ型胶原氨基端肽(PINP) |
| DOI：10.11724/jdmu.2003.02.07 |
| 分类号:R587.1 |
| 基金项目: |
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| Effect of insulin on bone formation in streptozotocin- induced diabetic rats |
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ZHANG Chun-yu1, WANG Bo2, Lv Shen1, LI Shu-fen1, WEN Li-rong1
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1.Second Affiliated Hospital of Dalian Medical University, Dalian 116027,China;2.Department of Pathology of Dalian Medical University, Dalian 116027, China
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| Abstract: |
| To determine the effect of insulin in inhibiting osteoporosis of streptozotocin-induced diabetic rats. ［Methods］Thirty 3-month-old male SD rats were divided randomly into three groups of 10 ，including diabetes (D), insulin treatment ( I ) and control (C) groups. Rats in group D and I were a dministered a single injection of streptozotocin to induce type 1 diabetes , while those in group C were physiological saline. Each rat in group I was given insulin twice a day (6-8u/d).On week 9 and 14， all rats were terminated,the lum |
| Key words: diabetic rats osteoporosis histomorphometry ins ulin PINP |