TNP-470和DDP联用对小鼠腹水瘤腹水生成的抑制作用

Inhibitory effect of the combination of TNP-470 and DDP on the ascitogenesis of ascitogenous tumor cells in mice

关键词:  血管生成抑制剂  TNP-470  DDP  腹水瘤细胞株  腹水

Key words:  angiogenesis inhibitor  TNP-470  DDP  ascitogenous tumor cell line  ascites

摘要:

为了验证0-(氯乙酰-氨甲酰基)烟曲霉醇（TNP-470）和顺铂（DDP）联用的增效作用。方法：将64只615小鼠腹腔内接种腹水瘤细胞株后，随机分为4组，然后腹腔内分别注射生理盐水（第1组），DDP（第2组），TNP-470（第3组）及联合用药（第4组），观察瘤细胞株对各组小鼠的腹水生成，瘤细胞数，存活率及生存时间。结果：DDP，TNP-470及联合用药组与生理盐水对照组相比可显著抑制615小鼠腹水的生成，减少瘤细胞数量，延长生存期（P<0.05）；联合用药组与其它3组比较在瘤细胞计数和瘤细胞存活率方面均有显著性差异（P<0.05）；在小鼠存活率及生存时间方面，联合用药组延长最明显；在用药过程中，联合用药组可引起体重下降，但停止用药后体重又可逐渐得到恢复。结论：TNP-470，DDP对小鼠腹水瘤腹腔种植后腹水的生成都有明显的抑制作用；TNP-470和DDP联用能起增效作用；体重下降这一副作用是可逆的。

Abstract:

To determine whether O-(chloroacetyl-carbamoyl)fumagillol(TNP-470) could potentiate the anti-tumor effect of cisplatin (DDP). Methods: Sixty-four inbred 615 mice were inoculated with ascitogenous tumor cells of HCa-P lines and were divided into 4 groups at random: Group 1, normal saline; Group 2, DDP; Group 3, TNP-470; Group 4, combination. Production of ascites, number of tumor cells, survival rate of tumor cells and survival period of mice were evaluated. Result: DDP, TNP-470 and combination obviously inhibited the production of ascites and reduced the number of tumor cells and prolonged the survival period of tumor-loading mice（P<0.05）.The number of tumor cells and survival rate of tumor cells of the combination group were significantly different from those of the other 3 groups（P<0.05）. The combination group was the most outstanding on the survival rate and the survival period of tumor-loading mice. During the period of administration, the combination group showed weight loss, without abnormal activity. After suspending administration, the weight could regain gradually. Conclusion: These results suggested that the combination of TNP-470 and DDP might produce additive effect. Although leading to weight loss, this was reversible