| 摘要: |
| 目的:研究巨噬细胞(Mφ)在榄香烯复合瘤苗(EC-TCV)对小鼠肝癌HCa-F的免疫保护效应中的应用.方法:榄香烯复合瘤苗主动免疫3次,体内用多聚甲醛固定的HCa-F致敏后3d检测腹腔巨噬细胞(Mφ)的抗肿瘤细胞毒活性、一氧化氮(NO)、细胞增殖活性的功能变化.结果:与对照组比较,腹腔巨噬细胞(PEMφ)对HCa-F的细胞毒活性增强,而在NO产生和细胞增殖方面无明显差异.结论:榄香烯复合瘤苗腹腔免疫可增强腹腔巨噬细胞的杀瘤活性. |
| 关键词: 巨噬细胞 榄香烯复合瘤苗 HCa-F 多聚甲醛 |
| DOI：10.11724/jdmu.2002.01.02 |
| 分类号:R73 |
| 基金项目:国家自然科学基金 |
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| The roles of macrophages in the immunoprotective effects of elemene combo-tumor cell vaccine against cancer in mice |
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SHEN Jie, GAO Shi-juan, GUO Lian-ying, SHI Guang-xia, QIAN Zhen-chao
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Department of Ｃancer Ｂiotherapy, Dalian Medical University, Dalian 116027，China
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| Abstract: |
| To study the roles of macrophages in the immunoprotective effects of elemene combo-tumor cell vaccine(EC-TCV) against HCa-F. Methods: The Balb/c inbred mice were immunized with EC-TCV and primed with HCa-F fixed with paraformaldehyde, the functions of the macrophages were measured on day 3. Results: Cytotoxicity against HCa-F of the peritoneal exudative macrophages(PE-Mφ)from the immunized mice was stronger than that of contral group and no significant difference in NO production and celluldur proliferation was seen between two groups. Conclusion: Intraperitoneal immunization of EC-TCV might induce cytotoxicity against cancer cells of HCa-F in mice |
| Key words: elemene combo-tumor vaccine macrophages HＣa-F paraformaldehyde |