榄香烯及榄香烯复合瘤苗对小鼠腹腔巨噬细胞功能影响的分析

Analysis of the effect of elemene and elemene combo-TCV on the functions of murine peritoneal exudative macroghages(PEMφ)

关键词:  巨噬细胞  榄香烯复合瘤苗  短小棒状杆菌

Key words:  macrophage  elemene combo-TCV  corynebacterium parvum

摘要:

分析小鼠腹腔巨噬细胞（peritoneal exudative macrophage, PEMφ）在榄香烯复合瘤苗免疫保护效应中的作用。方法：用血红蛋白释放法、硝酸还原法、MTT法等检测榄香烯及榄香烯复合瘤苗对小鼠腹腔巨噬细胞的吞噬、一氧化氮的产生、杀瘤以及对脂多糖（lipopolysaccharide，LPS）和粗提淋巴因子刺激的反应等功能的影响。结果：低浓度（5，0.5 μg/ml）的榄香烯和榄香烯复合瘤苗在体外可以增强PEMφ的活性，但瘤苗及其HSP70-肿瘤肽免疫后其PEMφ的上述功能未见变化。结论：榄香烯复合瘤苗的免疫保护效应并非通过PEMφ而实现。而短小棒状杆菌作为榄香烯复合瘤苗的佐剂，则可以明显增强PEMφ的功能

Abstract:

To analyse the function of the peritoneal exudative macroghages ( PEMφ) in the immunoprotective effect of elemene combo-TCV. Methods : we examined the effect of elemene and elemene combo-TCV on the functions of PEMφ including their cytotoxcity against tumor target cells by MTT assay, nitricoxide（NO）production by NO assay kit, phagocytosis activity by hemoglobin enzyme release assay (Hb-ERA) and their response to ipopolysaccharide（LPS）or crude lymphokine（LK）stimulation. Results: Low concentration（5, 0.5μg/ml）elemene and elemene combo-TCV could in vitro enhance the activity of PEMφ. But the functions of PEMφ obtained from TCVs and their HSP70-tumor peptide complexes immunized mice did not show significant change as compared with those of PEMφ from non-immunized mice. Conclucions: The active immunoprotective effect of the elemene combo-TCV was not achieved through PEMφ, while CP, as an adjuvant of elemene combo-TCV, could obviously increase the number and enhance the functions of PEMφ.