| 摘要: |
| 利用PCR及ELISA方法联合检测HBV在肝细胞癌肝组织中及血清标本中的感染情况。HBV-DNA在肝组织与血清标本中的检出率不同，分别为81.3%和37.5%。血清HBV-DNA的检出与HBsAg不一致。提示血清和肝组织中的HBV-DNA的差异检测结合ELISA可以初步显示HBV在体内的感染情况。 |
| 关键词: 乙型肝炎病毒 肝肿瘤 |
| DOI：10.11724/jdmu.2000.02.22 |
| 分类号:R446 |
| 基金项目: |
|
| Detection of HBV-DNA in liver tissue and serum of HCC patient |
|
ZHANG Sheng-liang,WEI Wei,ZHANG Yao-zheng
|
|
(Department of Clinical Biochemistry,Dalian Medical University,Dalian 116027,China
|
| Abstract: |
| HBV was detected in HCC patients' serum and liver tissue by using PCR and ELISA.The rate of HBV-DNA detected in liver tissue and serum were 81.3% and 37.5% respectively.The different results were determined between HBV-DNA and HBsAg in serum.These results suggested that the different types of HBV infection in HCC were determined more accurately by combining detection of HBV-DNA in different tissues and anti-HBV in serum |
| Key words: hepatitis B virus liver tumor |