| 摘要: |
| 介绍一种琼脂糖电泳法分离血清碱性磷酸酶肝型和骨型同工酶的改进方法。方法：将血清用神经氨酸苷酶处理，在此基础上选用四种缓冲液配制成0.7%的凝胶，加入1%的Triton X-100电泳，并进行荧光扫描。结果：四种缓冲液中巴比妥／HCL／EDTA缓冲液的分离效果最好。加入神经氨酸苷酶改变了肝型和骨型的迁移率，使电泳分离距增宽。加入Triton X-100使各区带结合更紧密，同工酶更易分辨。结论：采用合适的缓冲液体系，使用神经氨酸苷酶和Triton X-100作为辅助手段并用荧光扫描，使定量测定肝及骨型同工酶成为可能，且灵敏度和精密度令人满意。 |
| 关键词: 碱性磷酸酶同工酶 神经氨酸苷酶 琼脂糖电泳 |
| DOI：10.11724/jdmu.2000.02.23 |
| 分类号:R446.11 |
| 基金项目: |
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| Improved agarose gel electrophoretic method for seperating bone and liver ALP isoenzymes in serum |
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XIAO Xiao-guang
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(Department of Clinical Laboratory,The First Affiliated Hospital,Dalian Medical University,Dalian 116011,China)
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| Abstract: |
| To detect a new agarose gel electrophoretic method for separating bone and liver alkaline phosphatase isoenzymes in serum.Method:Four groups of buffers were choiced to made 0.7% of agarose and adding 1% Triton X-100 in it.For treating samples with neuraminidate before electrophoresis, then the samples were scanned by fluoresce.Results:barbital/HCL/EDTA was the best of the buffers.Adding neuraminidate was changed the migration of liver ALP and bone ALP,made the distance of separation wide.Adding Triton X-100 made the bands moretightness and isoenzymes was easy to identify.Conclusion:The method used the suitably buffer system, neuraminidate and Triton X-100,it made the quantitive analysis bone and liver ALP isoenzyme in serum become impossible and let us satisfied to sensitivity and precision. |
| Key words: ALP isoenzyme neuraminidate agarose gel electrophoretisis |