硝苯地平对内皮细胞和平滑肌细胞一氧化氮生成的影响

Nifedipine inhibits the production of nitric oxide in endothelial cells and smooth muscle cells in cutture

关键词:  硝苯地平  一氧化氮  内皮细胞  平滑肌细胞

Key words:  Nifedipine  nitric oxide  endothelial cells  smooth muscle cells

摘要:

钙离子通道拮抗药能够有效治疗某些心血管系统疾病.本实验研究了钙拮抗药硝苯地平对离体培养的内皮细胞及平滑肌细胞一氧化氮生成的影响.结果表明硝苯地平能够有效抑制上述细胞一氧化氮的释放,且抑制效应呈剂量依赖关系.提示钙拮抗药的治疗作用可能与其抑制一氧化氮的释放有关.

Abstract:

Calcium channel antagonists are effective in the treatment of some vascular diseases, such as hypertension,heart failure and various models of endotoxin shock.This study investigate effect of a calcium antagonist of nifedipine on the production of (NO) in cultured endothelial cells (CPAE cells,T529 cells)and smooth muscle cells (SM3 cells).The results demonstrated that nifedipine can inhibit the production of nitric oxide in T529 cells,CPAE cells and SM3 cells, and also inhibit the amount of NO stimulated by LPS in macrophages.The data suggest that some of the beneficial cardiovascular effect of treatment with calcium channel antagonists are due to inhibition of production of nitric oxide