凋亡细胞及过氧化物酶体增殖物激活受体γ在子痫前期患者胎盘中的表达及临床意义

Clinical significance of apoptosis cells and PPARγ expression in the placenta of patients with preeclampsia

关键词:  中间型滋养细胞  子痫前期  细胞凋亡  PPARγ

Key words:  intermediate trophoblast  preeclampsia  apoptosis  PPARγ

摘要:

目的　研究中间型滋养细胞的凋亡及过氧化物酶体增殖物激活受体γ（PPARγ）在子痫前期患者胎盘中的表达及临床意义。　方法　收集2014-2016年间大连市妇女儿童医疗中心住院分娩的正常妊娠晚期（正常组）、轻度子痫前期（轻度子痫前期组）及重度子痫前期（重度子痫前期组）产妇胎盘组织，各30例。采用 TUNEL法检测3组胎盘底板中间型滋养细胞的凋亡并计算凋亡指数（AI），采用免疫组化S-P法检测PPARγ的表达情况。　结果　正常妊娠晚期组、轻度子痫前期组及重度子痫前期组胎盘滋养细胞的AI分别为1.5860±0.2541、5.2609±2.8643及9.0000±6.2763，呈增高趋势，各组间差异有显著性意义（P<0.05）。PPARγ在正常妊娠晚期组、轻度子痫前期组及重度子痫前期组的表达阳性率分别为46.7%，93.3%，100%，呈增强趋势，各组间差异有显著性意义(P<0.05）。子痫前期胎盘组织中PPARγ与凋亡细胞的表达呈正相关(r0.591, P<0.05)。　结论　PPARγ可能通过诱导胎盘滋养细胞的凋亡参与子痫前期的发病。

Abstract:

Objective　To investigate the clinical significance of apoptosis index (AI) and PPARγ expression in the placenta of patients with preeclampsia. 　Methods　The placenta tissues from normal-term pregnancy, light preeclampsia and severe preeclampsia patients (30 cases in each group) were collected from 2014-2016 years in Dalian Municipal Women and Children，s Medical Center. TUNEL was performed to measure AI and immunohistochemistry (S-P method) was used to detect PPARγ expression in intermediate trophoblast of placental floor. 　Results　 AI was 1.5860±0.2541, 5.2609±2.8643 and 9.0000±6.2763 in normal-term pregnancy group, the light preeclampsia group and the severe preeclampsia group, respectively (P<0.05). The PPARγ expression frequency was 46.7%, 93.3% and 100% in normal-term pregnancy group, the light preeclampsia group and the severe preeclampsia group, respectively (P<0.05). The AI was positively correlated with PPARγ expression in placenta of patients with preeclampsia (P<0.05). 　Conclusion　PPARγ could involve in the pathogenesis of preeclampsia by inducing apoptosis of placental trophoblast cells.